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PMID: 7218437 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of supercoiled nucleoprotein complexes released from detergent-treated vaccinia virions.

Journal of virology ·Vol. 37 ·No. 2 ·1981-02-00 ·Pages 770-83

Soloski MJ, Holowczak JA

Abstract

Treatment of vaccinia virions with 1% sodium dodecyl sulfate in the absence of reducing agents resulted in the release of subviral particles termed "subnucleoids," which contained viral DNA in combination with four polypeptides with molecular weights of 90,000, 68,000, 58,000 and 10,000. Biochemical and electron microscopic studies showed that viral DNA in combination with these polypeptides was maintained in a superhelical configuration. When subnucleoids were "fixed" with glutaraldehyde and formaldehyde and then examined by electron microscopy, spherical particles were observed, in which the supercoiled DNA was folded into globular structures that were 20 to 60 nm in diameter and were interconnected by DNA-protein fibers resembling the nucleosome structures described for eucaryotic chromatin.

MeSH Terms
Centrifugation, Density Gradient DNA, Superhelical/isolation & purification DNA, Viral/isolation & purification Deoxyribonucleoproteins/isolation & purification Electrophoresis, Polyacrylamide Gel Ethidium Micrococcal Nuclease Microscopy, Electron Molecular Conformation Nucleoproteins/isolation & purification Sodium Dodecyl Sulfate Vaccinia virus/analysis,ultrastructure Viral Proteins/isolation & purification
Chemicals
DNA, Superhelical DNA, Viral Deoxyribonucleoproteins Nucleoproteins Viral Proteins Sodium Dodecyl Sulfate Micrococcal Nuclease Ethidium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Soloski M J
Holowczak J A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.37.2.770-783.1981
Published
1981-02-00
Pages
770-83
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC171065
Subset
IM
Grants
NCI NIH HHS · CA 11027 · United States
NCI NIH HHS · CA-09069 · United States
External Links
PubMed source DOI: 10.1128/JVI.37.2.770-783.1981 PMC Full Text View journal details
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