Transcriptional properties of nucleoli isolated from Tetrahymena.

Nucleic acids research ·Vol. 5 ·No. 11 ·1978-11-00 ·Pages 3993-4006

Gocke E, Leer JC, Nielsen OF, Westergaard O

Abstract

Nucleoli can be isolated from Tetrahymena in a yield of 30-60%. The isolated nucleoli contain rDNA (at least 90% pure) and have a protein to DNA ratio of 30:1. The endogenous RNA-polymerase activity of the r-chromatin has the following properties: (i) The in vitro transcript has a maximal size identical to the in vivo 35S rRNA precursor, demonstrating correct termination on the gene, (ii) 79% of the in vitro transcript is complementary to cDNA of 17S and 25S rRNA which is close to the theoretical maximum for the 35S rRNA precursor, (iii) the elongation rate of the endogenous RNA-polymerase molecules is 9-12 nucleotides/sec, (iv) an average of 4-16 active RNA polymerases are associated with each rDNA molecule depending upon the preparation.

MeSH Terms

Animals Cell Fractionation Cell Nucleolus/metabolism,ultrastructure Chromatin/metabolism DNA-Directed RNA Polymerases/metabolism Kinetics Nucleic Acid Hybridization Tetrahymena/metabolism Transcription, Genetic

Chemicals

Chromatin DNA-Directed RNA Polymerases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Gocke E

Leer J C

Nielsen O F

Westergaard O

References (26)

26 references, click to expand

A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
Biochem J. 1956 Feb;62(2):315-23 PMID: 13293190
Polols and turnover rates of nuclear ribosomal RNA in Tetrahymena pyriformis.
Eur J Biochem. 1970 Jul;14(3):460-4 PMID: 5479377
The sv40 transcription complex. I. Effect of viral chromatin proteins on endogenous RNA polymerase activity.
Nucleic Acids Res. 1977 Dec;4(12):4261-77 PMID: 202928
The mechanism by which heparin stimulates transcription in isolated rat liver nuclei. Polyribonucleotide elongation rates and the number of transcribing RNA polymerase molecules present.
Eur J Biochem. 1977 Oct 3;79(2):525-33 PMID: 923565
Properties of simian virus 40 transcriptional intermediates isolated from nuclei of permissive cells.
J Virol. 1977 Jul;23(1):20-8 PMID: 196103
Isolation of ribosomal gene chromatin.
Dev Biol. 1977 Feb;55(2):375-86 PMID: 557006
The sv40 transcription complex. II. Non-dissociation of protein from SV40 chromatin during transcription.
Nucleic Acids Res. 1977 Dec;4(12):4279-89 PMID: 202929
Fidelity of synthesis of preribosomal RNA in isolated nucleoli and nucleolar chromatin.
Proc Natl Acad Sci U S A. 1977 Jun;74(6):2446-50 PMID: 196290
Preparation of chromatin containing ribosomal deoxyribonucleic acid from the macronucleus of Tetrahymena pyriformis.
Biochem J. 1978 Jul 1;173(1):145-53 PMID: 99140
Introduction of hidden breaks during rRNA maturation and ageing in Tetrahymena pyriformis.
Eur J Biochem. 1978 Jul 3;87(3):607-16 PMID: 98329
Isolation of transcriptionally active chromatin from mammalian nucleoli.
Proc Natl Acad Sci U S A. 1977 Jan;74(1):69-73 PMID: 264695
Nucleolar chromatin in Chinese hamster ovary cells. Topographical distribution of ribosomal DNA sequences and isolation of ribosomal transcription complexes.
Eur J Biochem. 1977 Sep 15;79(1):23-32 PMID: 913418
Isolation of the ribosomal RNA gene from Tetrahymena in the state of transcriptionally active chromatin.
Biochem Biophys Res Commun. 1976 SEP 20;72(2):720-31 PMID: 825121
Simple agar--urea-gel electrophoretic fractionation of high molecular weight ribonucleic acids.
Anal Biochem. 1976 Nov;76(l):250-8 PMID: 793442
Animal DNA-dependent RNA polymerases. Analysis of the RNAs synthesized on Simian virus 40 superhelical DNA by mammalian RNA polymerases AI and B.
Eur J Biochem. 1974 Jan 16;41(2):379-95 PMID: 4361418
Quantitation of elongating form A and B RNA polymerases in chick oviduct nuclei and effects of estradiol.
Cell. 1976 Mar;7(3):455-65 PMID: 181146
Isolation and partial characterization of a nuclear RNA polymerase - SV40 DNA complex.
FEBS Lett. 1975 Aug 1;56(1):149-55 PMID: 169150
Comparison of the sequences of macro- and micronuclear DNA of Tetrahymena pyriformis.
Chromosoma. 1974;48(1):1-18 PMID: 4218159
Induction of nucleolar and mitochondrial DNA replication in Tetrahymena pyriformis.
Proc Natl Acad Sci U S A. 1974 Mar;71(3):894-8 PMID: 4207073
Electrophoresis of RNA in formamide.
Biochemistry. 1974 Dec 17;13(26):5373-8 PMID: 4611484
Free ribosomal RNA genes in the macronucleus of Tetrahymena.
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3078-81 PMID: 4528573
The cocoonase zymogen cells of silk moths: a model of terminal cell differentiation for specific protein synthesis.
Curr Top Dev Biol. 1972;7:125-91 PMID: 4643798
Free ribosomal DNA molecules from Tetrahymena pyriformis GL are giant palindromes.
J Mol Biol. 1976 Jun 25;104(2):455-70 PMID: 820864
The macronuclear ribosomal DNA of Tetrahymena pyriformis is a palindrome.
J Mol Biol. 1976 Jun 25;104(2):421-53 PMID: 820863
Synthesis of RNA molecules larger than 45 S by isolated rat-liver nucleoli.
Eur J Biochem. 1975 Sep 1;57(1):159-67 PMID: 1175640
Control of RNA transcription in nuclei and nucleoli of Physarum polycephalum.
FEBS Lett. 1978 Feb 15;86(2):303-6 PMID: 564291

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/5.11.3993

Published

1978-11-00

Pages

3993-4006

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342729

Subset

External Links

PubMed source DOI: 10.1093/nar/5.11.3993 PMC Full Text View journal details

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Transcriptional properties of nucleoli isolated from Tetrahymena.

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