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PMID: 727775 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Detection of staphylococcal enterotoxins A, B, C, D, and E in foods by radioimnunoassay, using staphyloccal cells containing protein A as immunoadsorbent.

Applied and environmental microbiology ·Vol. 36 ·No. 3 ·1978-09-00 ·Pages 421-6

Miller BA, Reiser RF, Bergdoll MS

Abstract

A sensitive radioimmunoassay utilizing Staphylococcus aureus cells containing protein A as a coprecipitant was developed for the detection and quantitation of staphylococcal enterotoxins A, B, C, D, and E in a variety of foods. The enterotoxins were extracted from the foods by a simple and rapid procedure. The sensitivity of the assay is 1.0 ng or less of enterotoxin per g of food.

MeSH Terms

Bacterial Toxins/analysis Enterotoxins/analysis Food Contamination/analysis Food Microbiology Immunosorbents Radioimmunoassay/methods Staphylococcal Protein A Staphylococcus/analysis

Chemicals

Bacterial Toxins Enterotoxins Immunosorbents Staphylococcal Protein A

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Miller B A

Reiser R F

Bergdoll M S

References (19)

19 references, click to expand

Iodination of staphylococcal enterotoxin B by use of chloramine-T.
Appl Environ Microbiol. 1977 Apr;33(4):824-8 PMID: 17366
Statistical analysis and quality control in radioimmunoassays for staphylococcal enterotoxins A, B, and C.
Appl Environ Microbiol. 1977 Dec;34(6):710-4 PMID: 74235
Preparation of labeled staphylococcal enterotoxin A with high specific activity.
Appl Environ Microbiol. 1976 Dec;32(6):735-40 PMID: 1008553
Double-antibody radioimmunoassay for staphylococcal enterotoxin C2.
Appl Microbiol. 1975 Oct;30(4):525-9 PMID: 1081374
Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.
J Immunol. 1975 Dec;115(6):1617-24 PMID: 1102604
Stability of 125I-labeled staphylococcal enterotoxins in solid-phase radioimmunoassay.
Appl Microbiol. 1975 Jun;29(6):776-9 PMID: 1155934
Staphylococcal enterotoxin C: solid-phase radioimmunoassay.
Appl Microbiol. 1975 Oct;30(4):700-1 PMID: 1190765
Standardization of the chloramine-T method of protein iodination.
Proc Soc Exp Biol Med. 1970 Mar;133(3):989-92 PMID: 4190944
A rapid solid phase radioimmunoassay for staphylococcal B enterotoxin.
J Immunol. 1972 Mar;108(3):852-6 PMID: 4622189
Rapid solid-phase radioimmunoassay for staphylococcal enterotoxin A.
Appl Microbiol. 1973 May;25(5):774-7 PMID: 4715556
Staphylococcal enterotoxins A and B: solid-phase radioimmunoassay in food.
Appl Microbiol. 1973 Sep;26(3):309-13 PMID: 4796162
Detection of staphylococcal enterotoxin in foods.
Appl Microbiol. 1974 Jan;27(1):83-5 PMID: 4809910
Ammonium sulfate coprecipitation antibody determination with purified staphylococcal enterotoxins.
J Bacteriol. 1969 Jul;99(1):18-24 PMID: 4979439
Staphylococcal enterotoxin B: solid-phase radioimmunoassay.
Appl Microbiol. 1971 Nov;22(5):837-41 PMID: 5002312
Enzymatic iodination of polypeptides with 125I to high specific activity.
Biochim Biophys Acta. 1971 Dec 28;251(3):363-9 PMID: 11452877
Insulin-I131 metabolism in human subjects: demonstration of insulin binding globulin in the circulation of insulin treated subjects.
J Clin Invest. 1956 Feb;35(2):170-90 PMID: 13286336
THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.
Biochem J. 1963 Oct;89:114-23 PMID: 14097352
DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN IN FOOD.
Appl Microbiol. 1965 Mar;13:181-9 PMID: 14325876
Modified radioimmunoassay determination for staphylococcal enterotoxin B in foods.
Appl Environ Microbiol. 1977 Mar;33(3):620-5 PMID: 16345216

Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.36.3.421-426.1978

Published

1978-09-00

Pages

421-6

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC243063

Subset

External Links

PubMed source DOI: 10.1128/aem.36.3.421-426.1978 PMC Full Text View journal details

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Detection of staphylococcal enterotoxins A, B, C, D, and E in foods by radioimnunoassay, using staphyloccal cells containing protein A as immunoadsorbent.

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