Studies on human pure pancreatic juice collected by endoscopic retrograde catheterization of the papilla.

Gastroenterologia Japonica ·Vol. 13 ·No. 5 ·1978-00-00 ·Pages 383-9

Harada H, Ono A, Yamamoto N, Ikubo I, Negron A, Hayashi T, Sato T, Mishima K, Kimura I

Abstract

Pure pancreatic juice was collected from 8 subjects by endoscopic retrograde cannulation of the papilla. Samples were collected at 1 minute intervals for 20 minutes after rapid intravenous injection of secretin (Eisai, 1u/kg) and for 10 to 15 minutes after rapid intravenous injection of CCK-PZ (1 U/kg). Three hydrolases (amylase, chymotrypsinogen and lipase) showed nonparallel secretory pattern. Lipase was proportionally more stimulated than chymotrypsinogen and chymotrypsinogen was more stimulated than amylase by CCK-PZ stimulation. However, in one subject the secretion of three hydrolases was parallel and in another subject lipase and chymotrypsinogen showed parallelism. Samples collected at "wash out" period showed quite high enzyme and protein concentration with specific activity and enzyme ratio not different from those of samples collected at peak period of CCK-PZ stimulation. Normal ranges were estimated in peak flow rate per minute, peak enzyme concentration, specific activity of enzymes, maximal enzyme output per minute and maximal bicarbonate concentration.

MeSH Terms

Amylases/metabolism Catheterization/methods Cholecystokinin/pharmacology Chymotrypsinogen/metabolism Endoscopy/methods Humans Lipase/metabolism Pancreatic Ducts Pancreatic Juice/enzymology,metabolism Specimen Handling/methods

Chemicals

Cholecystokinin Chymotrypsinogen Lipase Amylases

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Harada H

Ono A

Yamamoto N

Ikubo I

Negron A

Hayashi T

Sato T

Mishima K

Kimura I

References (20)

20 references, click to expand

[A new test of exocrine pancreatic function using N-benzoyl-L-tyrosyl-p-aminobenzoic acid. Part II. The correlation of PFD and chymotrypsin output during PS-test (author's transl)].
Nihon Shokakibyo Gakkai Zasshi. 1977 Oct;74(10):1337-46 PMID: 304114
The interrelationships of pancreatic enzymes in human duodenal aspirate.
Gut. 1970 Oct;11(10):859-66 PMID: 5485836
Letter: Does the pancreas secrete digestive enzymes individually or in a group?
Gastroenterology. 1976 Apr;70(4):635-6 PMID: 1254150
Molecular regulation of digestion: short term and bond specific.
Am J Physiol. 1974 Jan;226(1):77-83 PMID: 4809890
Pancreatic secretion of total protein and of three hydrolases collected in healthy subjects via duodenoscopic cannulation. Effects of secretin, pancreozymin, and caerulein.
Gastroenterology. 1975 Aug;69(2):374-9 PMID: 1150045
[Studies of the maximum exzymatic capacity of the normal human exocrein pancreas during continous infusion of secretin and pancreozymin].
Biol Gastroenterol (Paris). 1968;2:163-70 PMID: 5746394
Pancreatic exocrine secretion in normal subjects studied by continuous infusion of pancreozymin and secretin.
Scand J Gastroenterol. 1969;4(7):597-602 PMID: 5383686
The interrelationships of pancreatic enzymes in health and diseases under cholecystokinin stimulation.
Digestion. 1973;9(1):8-20 PMID: 4751322
A test of pancreatic function in man based on the analysis of duodenal contents after administration of secretin and pancreozymin.
Gut. 1960 Jun;1:111-24 PMID: 13806339
Chymodenin, a duodenal peptide: specific stimulation of chymotrypsinogen secretion.
Am J Physiol. 1975 Dec;229(6):1680-6 PMID: 1211501
Nonparallel secretion of enzymes in human duodenal juice and pure pancreatic juice collected by endoscopic retrograde catheterization of the papilla.
Gastroenterology. 1977 Jul;73(1):42-5 PMID: 863199
Relationship between the structures of S-acyl thiol compounds and their rates of hydrolysis by pancreatic lipase and hepatic carboxylic esterase.
J Biochem. 1976 Mar;79(3):533-41 PMID: 985813
Ratio of chymotrypsin to trypsin in human duodenal aspirate.
Digestion. 1973;8(2):101-9 PMID: 4709596
[Different behavior of lipase, amylase and pancreatic proteolytic enzymes after different means of excitation of the human pancreas].
Bull Soc Chim Biol (Paris). 1966;48(8):951-7 PMID: 5924594
A novel and simple colorimetric assay for human serum lipase.
J Biochem. 1977 Feb;81(2):361-9 PMID: 14943
[STUDY OF REPEATED INJECTIONS AND CONTINUOUS PERFUSION OF SECRETIN IN HUMANS].
Arch Mal Appar Dig Mal Nutr. 1965 Mar;54:177-94 PMID: 14303320
A secretin test with high doses of secretin and correction for incomplete recovery of duodenal juice.
Gastroenterology. 1967 Jan;52(1):67-77 PMID: 6018374
Exocrine pancreas: evidence for topographic partition of secretory function.
Science. 1975 Nov 21;190(4216):795-7 PMID: 1105788
The interrelationships of the pancreatic enzymes.
Gut. 1972 May;13(5):398-412 PMID: 4556424
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713

Article Info

Journal

Gastroenterologia Japonica

Abbr.

Gastroenterol Jpn

ISSN

0435-1339

DOI

10.1007/BF02776015

Published

1978-00-00

Pages

383-9

Language

English

Region

Japan

NLM ID

0152744

Subset

External Links

PubMed source DOI: 10.1007/BF02776015

Analysis Services

Studies on human pure pancreatic juice collected by endoscopic retrograde catheterization of the papilla.

Contact

WeChat Official Account