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PMID: 7309227 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In vitro formation of hybrid toxins between subunits of Escherichia coli heat-labile enterotoxin and those of cholera enterotoxin.

Infection and immunity ·Vol. 34 ·No. 2 ·1981-11-00 ·Pages 341-6

Takeda Y, Honda T, Taga S, Miwatani T

Abstract

Heat-labile enterotoxin (LT) was purified from cells of enterotoxigenic Escherichia coli isolated from a patient with traveller's diarrhea. Purified LT was separated into A and B subunits by treatment with 6 M urea solution in 0.1 M propionic acid (pH 4.0). Biologically active toxin was reconstituted from isolated A and B subunits of LT. Hybrid toxins with biological activity were obtained in vitro from the A subunit of cholera enterotoxin and B subunit of LT, and from the A subunit of LT and B subunit of cholera enterotoxin. The hybrid toxins show a similar toxicity to that of the parent toxins from which the A subunits were derived. The in vitro formations of the hybrid toxins were confirmed by polyacrylamide gel disk electrophoresis.

MeSH Terms
Animals Bacterial Toxins Biological Assay Chemical Phenomena Chemistry Cholera Toxin/isolation & purification,toxicity Cricetinae Electrophoresis, Polyacrylamide Gel Enterotoxins/isolation & purification,toxicity Escherichia coli Proteins Rabbits
Chemicals
Bacterial Toxins Enterotoxins Escherichia coli Proteins heat-labile enterotoxin, E coli Cholera Toxin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Takeda Y
Honda T
Taga S
Miwatani T
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27 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.34.2.341-346.1981
Published
1981-11-00
Pages
341-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC350871
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.34.2.341-346.1981 PMC Full Text View journal details
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