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PMID: 1033156 Published · ppublish English Journal Article

Isolation of a factor causing morphological changes of chinese hamster ovary cells from the culture filtrate of Vibrio parahaemolyticus.

Infection and immunity ·Vol. 14 ·No. 4 ·1976-10-00 ·Pages 1028-33

Honda T, Shimizu M, Takeda Y, Miwatani T

Abstract

A factor changing Chinese hamster ovary cells from an oval to a spindle shape was isolated from the culture filtrate of Vibrio parahaemolyticus. It was partially purified by successive column chromatographies on diethylaminoethylcellulose, diethylaminoethyl-Sephadex A-25, hydroxylapatite, and Sephadex G-200. This factor was separated from thermostabl direct hemolysin and was heat labile.

MeSH Terms
Animals Bacterial Toxins/analysis,isolation & purification Cell-Free System Cells, Cultured Cricetinae Female Filtration Hot Temperature Ovary/cytology,microbiology Vibrio parahaemolyticus
Chemicals
Bacterial Toxins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Honda T
Shimizu M
Takeda Y
Miwatani T
References (21)
21 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.14.4.1028-1033.1976
Published
1976-10-00
Pages
1028-33
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC415489
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.14.4.1028-1033.1976 PMC Full Text View journal details
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