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PMID: 7396854 Published · ppublish English Journal Article

Isolation and characterization of the thymus-brain antigen (analogous to thy-1 antigen) from human brain.

The Biochemical journal ·Vol. 187 ·No. 2 ·1980-05-01 ·Pages 403-12

Hamann A, Arndt R, Klein P, Thiele HG

Abstract

1. The human thymus-brain antigen, which corresponds to the murine (mouse or rat) Thy-1 antigen complex, was isolated from brain after solubilization in deoxycholate by gel-permeation chromatography, wheat-germ-lectin affinity chromatography and ion-exchange chromatography. 2. The isolated antigen is a glycoprotein displaying an apparent molecular weight of 26 000-29 000 in sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. 3. No antigen activity was found with the lipid fraction from human brain. 4. The protein has a tendency for spontaneous self-association (dimerization), leading to aggregates resistant to dissociating and reducing agents on prolonged storage. 5. The antigen is microheterogeneous with respect to size, charge (approximate isoelectric points of the monomer 7.7, 7.0 and 6.5) and to lectin-binding affinity. 6. The antigen can be reconstituted to protein-lipid vesicles. The antigen activity of solubilized antigen is strongly increased by reconstitution and that of membranes decreased by solubilization with detergent.

MeSH Terms
Antigens/isolation & purification Brain/immunology Centrifugation, Density Gradient Chemical Phenomena Chemistry Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Humans Macromolecular Substances Phospholipids Thymus Gland/immunology
Chemicals
Antigens Macromolecular Substances Phospholipids
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hamann A
Arndt R
Klein P
Thiele H G
References (38)
38 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1870403
Published
1980-05-01
Pages
403-12
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1161806
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1870403 PMC Full Text View journal details
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