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PMID: 7425617 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Development of ethanol tolerance in Clostridium thermocellum: effect of growth temperature.

Applied and environmental microbiology ·Vol. 40 ·No. 3 ·1980-09-00 ·Pages 571-7

Herrero AA, Gomez RF

Abstract

The growth of Clostridium thermocellum ATCC 27405 and of C9, an ethanol-resistant mutant of this strain, at different ethanol concentrations and temperatures was characterized. After ethanol addition, cultures continued to grow for 1 to 2 h at rates similar to those observed before ethanol was added and then entered a period of growth arrest, the duration of which was a function of the age of inocula. After this period, cultures grew at an exponential rate that was a function of ethanol concentration. The wild-type strain showed a higher energy of activation for growth than the ethanol-tolerant derivative. The optimum growth temperature of the wild type decreased as the concentration of the ethanol challenge increased, whereas the optimum growth temperature for C9 remained constant. The results are discussed in terms of what is known about the effects of ethanol and temperature on membrane composition and fluidity.

MeSH Terms
Clostridium/drug effects,genetics,growth & development Drug Tolerance Ethanol/pharmacology Mutation Temperature
Chemicals
Ethanol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Herrero A A
Gomez R F
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25 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.40.3.571-577.1980
Published
1980-09-00
Pages
571-7
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC291623
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.40.3.571-577.1980 PMC Full Text View journal details
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