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PMID: 7487495 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The US3 protein kinase of herpes simplex virus type 2 is associated with phosphorylation of the UL12 alkaline nuclease in vitro.

Archives of virology ·Vol. 140 ·No. 9 ·1995-00-00 ·Pages 1637-44

Daikoku T, Yamashita Y, Tsurumi T, Nishiyama Y

Abstract

Herpes simplex virus type 2 (HSV-2) gene US3 encodes a serine-threonine protein kinase. We previously described the isolation of a US3-inactivated mutant which is able to replicate in Vero cells but not in murine macrophages. To learn more about the biological role of the US3 protein kinase, we have sought to identify the target proteins of the enzyme. Studies of in vitro phosphorylation with extracts of infected cells demonstrate that the US3 protein kinase is involved in phosphorylation of the UL12 alkaline nuclease in vitro, suggesting that the nuclease is a possible target of the protein kinase.

MeSH Terms
Animals Cell Line Chlorocebus aethiops Mice Molecular Weight Phosphoproteins/chemistry,metabolism Phosphorylation Protein Serine-Threonine Kinases/metabolism Ribonucleases/metabolism Simplexvirus/enzymology Vero Cells Viral Proteins
Chemicals
Phosphoproteins Viral Proteins Protein Serine-Threonine Kinases US3 protein, Human herpesvirus 2 Ribonucleases viral alkaline nuclease
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Daikoku T
Laboratory of Virology, Nagoya University School of Medicine, Japan.
Yamashita Y
Tsurumi T
Nishiyama Y
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20 references, click to expand
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Article Info
Journal
Archives of virology
Abbr.
Arch Virol
ISSN
0304-8608
DOI
10.1007/BF01322537
Published
1995-00-00
Pages
1637-44
Language
English
Region
Austria
NLM ID
7506870
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01322537 View journal details
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