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PMID: 7490283 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Binding between the neural cell adhesion molecules axonin-1 and Nr-CAM/Bravo is involved in neuron-glia interaction.

The Journal of cell biology ·Vol. 131 ·No. 4 ·1995-11-00 ·Pages 1067-81

Suter DM, Pollerberg GE, Buchstaller A, Giger RJ, Dreyer WJ, Sonderegger P

Abstract

Neural cell adhesion molecules of the immunoglobulin superfamily mediate cellular interactions via homophilic binding to identical molecules and heterophilic binding to other family members or structurally unrelated cell-surface glycoproteins. Here we report on an interaction between axonin-1 and Nr-CAM/Bravo. In search for novel ligands of axonin-1, fluorescent polystyrene microspheres conjugated with axonin-1 were found to bind to peripheral glial cells from dorsal root ganglia. By antibody blockage experiments an axonin-1 receptor on the glial cells was identified as Nr-CAM. The specificity of the interaction was confirmed with binding studies using purified axonin-1 and Nr-CAM. In cultures of dissociated dorsal root ganglia antibodies against axonin-1 and Nr-CAM perturbed the formation of contacts between neurites and peripheral glial cells. Together, these results implicate a binding between axonin-1 of the neuritic and Nr-CAM of the glial cell membrane in the early phase of axon ensheathment in the peripheral nervous system.

MeSH Terms
Animals Antibody Specificity Axons/physiology Base Sequence Cell Adhesion/physiology Cell Adhesion Molecules Cell Adhesion Molecules, Neuronal/immunology,metabolism Chick Embryo Contactin 2 DNA Primers/chemistry Ganglia, Spinal/cytology,embryology Integrins/physiology Microspheres Molecular Sequence Data Neurites/ultrastructure Neuroglia/cytology,ultrastructure Neurons/cytology,ultrastructure Protein Binding/physiology
Chemicals
CNTN2 protein, human Cell Adhesion Molecules Cell Adhesion Molecules, Neuronal Contactin 2 DNA Primers Integrins NRCAM protein, human
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Suter D M
Institute of Biochemistry, University of Zurich, Switzerland.
Pollerberg G E
Buchstaller A
Giger R J
Dreyer W J
Sonderegger P
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.131.4.1067
Published
1995-11-00
Pages
1067-81
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2200008
Subset
IM
Grants
NEI NIH HHS · EY07725 · United States
External Links
PubMed source DOI: 10.1083/jcb.131.4.1067 PMC Full Text View journal details
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