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PMID: 7512160 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A domain of murine retrovirus surface protein gp70 mediates cell fusion, as shown in a novel SC-1 cell fusion system.

Journal of virology ·Vol. 68 ·No. 5 ·1994-05-00 ·Pages 3175-82

Andersen KB

Abstract

Virus-induced cell fusion of the fusion-from-without type was observed in SC-1 cells infected with Moloney murine leukemia virus when grown in NIH 3T3 cells. Replication-competent virus mutants with altered surface protein gp70 were examined. Fusion mutations were found in the proline-rich region of gp70. They acted on a step after binding and before or during endocytosis. The fusion mutants had an altered gp70 isomer pattern, presumably caused by different glycosylation. Other mutants with deleted glycans were analyzed, and some which also showed defective fusion were found. The interrelationship of the proline-rich region, glycosylation, and fusion is discussed.

MeSH Terms
3T3 Cells Acute-Phase Proteins/physiology Animals Antibodies, Viral/pharmacology Cell Fusion/drug effects,physiology DNA Mutational Analysis Endocytosis Glycoproteins/physiology Glycosylation Mice Moloney murine leukemia virus/physiology Mutation Polysaccharides/physiology Retroviridae Proteins, Oncogenic/immunology,physiology Viral Envelope Proteins/immunology,physiology
Chemicals
Acute-Phase Proteins Antibodies, Viral Glycoproteins Polysaccharides Retroviridae Proteins, Oncogenic Viral Envelope Proteins
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Andersen K B
Department of Biology, Danish Royal School of Pharmacy, Copenhagen.
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.68.5.3175-3182.1994
Published
1994-05-00
Pages
3175-82
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC236808
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.68.5.3175-3182.1994 PMC Full Text View journal details
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