Home LiteratureArticle Details
PMID: 7512973 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

BEHAB, a new member of the proteoglycan tandem repeat family of hyaluronan-binding proteins that is restricted to the brain.

The Journal of cell biology ·Vol. 125 ·No. 2 ·1994-04-00 ·Pages 495-509

Jaworski DM, Kelly GM, Hockfield S

Abstract

Hyaluronan (HA) is a ubiquitous component of the extracellular matrix of all tissues. In the mammalian central nervous system (CNS) HA is present throughout development and into adulthood. While the functions of HA are likely to be mediated by HA-binding proteins, no cell or tissue specific HA-binding proteins have been reported. In an effort to characterize the composition of the extracellular matrix of the CNS, we sought to identify neural HA-binding proteins. We report here the isolation and characterization of a cDNA with a high degree of sequence homology to members of the proteoglycan tandem repeat (PTR) family of HA-binding proteins. Unlike other HA-binding proteins, the expression of this cDNA is restricted to the CNS. We propose the name BEHAB, Brain Enriched HyAluronan Binding protein, for this gene. The expression of BEHAB mRNA is developmentally regulated; expression is first detected in the late embryonic period and peaks during the first two postnatal weeks. In the embryo, BEHAB is expressed at highest levels in mitotically active cells. The sequence of BEHAB has long stretches of identity between rat and cat, suggesting that the encoded protein is functionally important. The size and sequence of BEHAB are consistent with the possibility that it could serve a function like link protein, stabilizing interactions between HA and brain proteoglycans. These observations suggest that existence of other tissue specific HA-binding proteins.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Brain/embryology,metabolism Brevican Carrier Proteins/genetics,metabolism Cats Chondroitin Sulfate Proteoglycans DNA Extracellular Matrix/metabolism Gene Expression Regulation Hyaluronan Receptors Hyaluronic Acid/metabolism In Situ Hybridization Lectins, C-Type Molecular Sequence Data Nerve Tissue Proteins/genetics,metabolism Proteoglycans/metabolism Rats Receptors, Cell Surface/genetics,metabolism Receptors, Lymphocyte Homing/genetics,metabolism Sequence Homology, Amino Acid
Chemicals
Bcan protein, rat Brevican Carrier Proteins Chondroitin Sulfate Proteoglycans Hyaluronan Receptors Lectins, C-Type Nerve Tissue Proteins Proteoglycans Receptors, Cell Surface Receptors, Lymphocyte Homing Hyaluronic Acid DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jaworski D M
Yale University School of Medicine, Section of Neurobiology, New Haven, Connecticut 06510.
Kelly G M
Hockfield S
References (71)
71 references, click to expand
  1. Cloning and primary structure of neurocan, a developmentally regulated, aggregating chondroitin sulfate proteoglycan of brain.
    J Biol Chem. 1992 Sep 25;267(27):19536-47 PMID: 1326557
  2. Characterization of multiple adhesive and counteradhesive domains in the extracellular matrix protein cytotactin.
    J Cell Biol. 1992 Nov;119(3):663-78 PMID: 1383239
  3. A large chondroitin sulfate proteoglycan has the characteristics of a general extracellular matrix component of adult brain.
    J Neurosci. 1993 Jan;13(1):195-207 PMID: 7678642
  4. Versican, a hyaluronate-binding proteoglycan of embryonal precartilaginous mesenchyma, is mainly expressed postnatally in rat brain.
    J Neurosci Res. 1993 Jan;34(1):97-106 PMID: 7678658
  5. Nervous tissue proteoglycans.
    Experientia. 1993 May 15;49(5):429-46 PMID: 8500598
  6. Identification of hyaluronic acid binding sites in the extracellular domain of CD44.
    J Cell Biol. 1993 Jul;122(1):257-64 PMID: 8314845
  7. A brain extracellular matrix proteoglycan forms aggregates with hyaluronan.
    J Biol Chem. 1993 Jul 15;268(20):15061-9 PMID: 8325882
  8. Calcium-dependent bacteriophage DNA infection.
    J Mol Biol. 1970 Oct 14;53(1):159-62 PMID: 4922220
  9. Histochemical demonstration of sulphated polysaccharides at the surface coat of nerve cells in the mouse central nervous system.
    Acta Histochem. 1970;38(1):55-64 PMID: 4993426
  10. Glycosaminoglycans of brain during development.
    Biochemistry. 1975 Jan 14;14(1):85-8 PMID: 122810
  11. Cartilage proteoglycans. Structure and heterogeneity of the protein core and the effects of specific protein modifications on the binding to hyaluronate.
    Biochem J. 1976 Jul 1;157(1):127-43 PMID: 134698
  12. The nature and metabolism of the carbohydrate-peptide linkages of glycoproteins.
    Biochem Soc Symp. 1974;(40):17-26 PMID: 4620382
  13. Decrease in levels and rates of synthesis of tubulin and actin in developing rat brain.
    Brain Res. 1977 Feb;121(2):327-42 PMID: 832166
  14. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  15. Extracellular matrix during laminar pattern formation of neocortex in normal and reeler mutant mice.
    Dev Biol. 1983 Feb;95(2):305-16 PMID: 6186545
  16. A surface antigen expressed by a subset of neurons in the vertebrate central nervous system.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5758-61 PMID: 6193523
  17. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  18. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  19. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
    Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
  20. Genomic sequencing.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 PMID: 6326095
  21. A surface antigen that identifies ocular dominance columns in the visual cortex and laminar features of the lateral geniculate nucleus.
    Cold Spring Harb Symp Quant Biol. 1983;48 Pt 2:877-89 PMID: 6426850
  22. Immunocytochemical localization of a chondroitin sulfate proteoglycan in nervous tissue. II. Studies in developing brain.
    J Cell Biol. 1984 Sep;99(3):1130-9 PMID: 6381505
  23. Specific chemical modifications of link protein and their effect on binding to hyaluronate and cartilage proteoglycan.
    Biochim Biophys Acta. 1986 Mar 19;881(1):22-9 PMID: 3081049
  24. The primary structure of link protein from rat chondrosarcoma proteoglycan aggregate.
    J Biol Chem. 1986 Mar 15;261(8):3519-35 PMID: 2419334
  25. The brain-specific gene 1B236 is expressed postnatally in the developing rat brain.
    J Neurosci. 1986 Feb;6(2):522-30 PMID: 3950709
  26. A new method for predicting signal sequence cleavage sites.
    Nucleic Acids Res. 1986 Jun 11;14(11):4683-90 PMID: 3714490
  27. Link protein cDNA sequence reveals a tandemly repeated protein structure.
    Proc Natl Acad Sci U S A. 1986 Jun;83(11):3761-5 PMID: 3459153
  28. A nerve terminal anchorage protein from electric organ.
    J Cell Biol. 1986 Aug;103(2):509-20 PMID: 2426282
  29. An unexpected sequence homology between link proteins of the proteoglycan complex and immunoglobulin-like proteins.
    Biochim Biophys Acta. 1986 Sep 5;873(1):152-5 PMID: 3741879
  30. Brain-specific hyaluronate-binding protein. A product of white matter astrocytes?
    J Neurocytol. 1986 Oct;15(5):671-9 PMID: 2430070
  31. Fibronectin-like immunoreactivity in the developing cerebral cortex.
    J Neurosci. 1987 Oct;7(10):3325-33 PMID: 3668630
  32. Immunoreactivity for laminin in the developing ventral longitudinal pathway of the brain.
    Dev Biol. 1988 Jan;125(1):135-44 PMID: 3334714
  33. The tandemly repeated sequences of cartilage link protein contain the sites for interaction with hyaluronic acid.
    J Cell Biol. 1987 Nov;105(5):2403-8 PMID: 2445761
  34. Complete primary structure of the rat cartilage proteoglycan core protein deduced from cDNA clones.
    J Biol Chem. 1987 Dec 25;262(36):17757-67 PMID: 3693370
  35. The immunoglobulin superfamily--domains for cell surface recognition.
    Annu Rev Immunol. 1988;6:381-405 PMID: 3289571
  36. Transient expression of laminin in the optic nerve of the developing rat.
    J Neurosci. 1988 Jun;8(6):1981-90 PMID: 3385486
  37. p1B15: a cDNA clone of the rat mRNA encoding cyclophilin.
    DNA. 1988 May;7(4):261-7 PMID: 3293952
  38. A lymphocyte molecule implicated in lymph node homing is a member of the cartilage link protein family.
    Cell. 1989 Mar 24;56(6):1057-62 PMID: 2466575
  39. Isolation and partial characterization of a glial hyaluronate-binding protein.
    J Biol Chem. 1989 Apr 5;264(10):5981-7 PMID: 2466833
  40. Extracellular matrix molecules that influence neural development.
    Annu Rev Neurosci. 1989;12:491-516 PMID: 2648958
  41. Immunoelectron microscopic localization of hyaluronic acid-binding region and link protein epitopes in brain.
    J Cell Biol. 1989 May;108(5):1899-907 PMID: 2469681
  42. Immunoglobulin fold and tandem repeat structures in proteoglycan N-terminal domains and link protein.
    J Mol Biol. 1989 Apr 20;206(4):737-53 PMID: 2738916
  43. A monoclonal antibody identifies a novel epitope surrounding a subpopulation of the mammalian central neurons.
    Neuroscience. 1989;29(3):645-57 PMID: 2472579
  44. Monoclonal antibodies to human lymphocyte homing receptors define a novel class of adhesion molecules on diverse cell types.
    J Cell Biol. 1989 Aug;109(2):927-37 PMID: 2474557
  45. Isolation of the N-terminal globular protein domains from cartilage proteoglycans. Identification of G2 domain and its lack of interaction with hyaluronate and link protein.
    Biochem J. 1989 Aug 1;261(3):801-9 PMID: 2803245
  46. Multiple domains of the large fibroblast proteoglycan, versican.
    EMBO J. 1989 Oct;8(10):2975-81 PMID: 2583089
  47. Inhibition of neural crest cell migration by aggregating chondroitin sulfate proteoglycans is mediated by their hyaluronan-binding region.
    Dev Biol. 1990 Jan;137(1):1-12 PMID: 1688536
  48. Glycosaminoglycan-related epitopes surrounding different subsets of mammalian central neurons.
    Neurosci Res. 1989 Nov;7(2):117-30 PMID: 2482466
  49. Molecular and cellular characterization of the glial roof plate of the spinal cord and optic tectum: a possible role for a proteoglycan in the development of an axon barrier.
    Dev Biol. 1990 Apr;138(2):359-76 PMID: 1690673
  50. Hyaluronate can function as a cell adhesion molecule and CD44 participates in hyaluronate recognition.
    J Exp Med. 1990 Jul 1;172(1):69-75 PMID: 2193100
  51. A diverse set of developmentally regulated proteoglycans is expressed in the rat central nervous system.
    Neuron. 1990 Jun;4(6):949-61 PMID: 2361016
  52. Complete coding sequence and deduced primary structure of the human cartilage large aggregating proteoglycan, aggrecan. Human-specific repeats, and additional alternatively spliced forms.
    J Biol Chem. 1991 Jan 15;266(2):894-902 PMID: 1985970
  53. Monoclonal antibody Cat-301 identifies Y-cells in the dorsal lateral geniculate nucleus of the cat.
    J Comp Neurol. 1990 Oct 15;300(3):320-30 PMID: 2266189
  54. The hyaluronate receptor is a member of the CD44 (H-CAM) family of cell surface glycoproteins.
    J Cell Biol. 1990 Dec;111(6 Pt 1):2765-74 PMID: 1703543
  55. Extracellular matrix molecules and their receptors: functions in neural development.
    Annu Rev Neurosci. 1991;14:531-70 PMID: 1851608
  56. Characterization of a hyaluronic acid-binding protein from sheep brain comparison with human brain hyaluronectin.
    Int J Biochem. 1991;23(3):329-37 PMID: 1710584
  57. Isolation and characterization of developmentally regulated chondroitin sulfate and chondroitin/keratan sulfate proteoglycans of brain identified with monoclonal antibodies.
    J Biol Chem. 1991 Aug 5;266(22):14785-801 PMID: 1907283
  58. Chondroitin sulfate proteoglycan surrounds a subset of human and rat CNS neurons.
    J Neurosci Res. 1991 Jun;29(2):225-34 PMID: 1890701
  59. Interaction of a brain extracellular matrix protein with hyaluronic acid.
    Biochim Biophys Acta. 1991 Oct 31;1075(3):248-58 PMID: 1720020
  60. An analysis of vertebrate mRNA sequences: intimations of translational control.
    J Cell Biol. 1991 Nov;115(4):887-903 PMID: 1955461
  61. Changes in the distribution of extracellular matrix components accompany early morphogenetic events of mammalian cortical development.
    J Neurosci. 1991 Dec;11(12):3928-42 PMID: 1720815
  62. Identification of a developmentally regulated keratan sulfate proteoglycan that inhibits cell adhesion and neurite outgrowth.
    Neuron. 1991 Dec;7(6):1007-18 PMID: 1764241
  63. Expression of neural proteoglycans correlates with the acquisition of mature neuronal properties in the mammalian brain.
    Cold Spring Harb Symp Quant Biol. 1990;55:505-14 PMID: 2132834
  64. A novel secretory tumor necrosis factor-inducible protein (TSG-6) is a member of the family of hyaluronate binding proteins, closely related to the adhesion receptor CD44.
    J Cell Biol. 1992 Jan;116(2):545-57 PMID: 1730767
  65. Proteoglycans: many forms and many functions.
    FASEB J. 1992 Feb 1;6(3):861-70 PMID: 1740236
  66. Hyaluronan.
    FASEB J. 1992 Apr;6(7):2397-404 PMID: 1563592
  67. Hyaluronate binding properties of versican.
    J Biol Chem. 1992 May 15;267(14):10003-10 PMID: 1577773
  68. The high molecular weight Cat-301 chondroitin sulfate proteoglycan from brain is related to the large aggregating proteoglycan from cartilage, aggrecan.
    J Biol Chem. 1992 May 15;267(14):9874-83 PMID: 1374409
  69. A chondroitin sulfate proteoglycan that is developmentally regulated in the cerebellar mossy fiber system.
    Dev Biol. 1992 Jun;151(2):564-74 PMID: 1376288
  70. Molecular cloning of a novel hyaluronan receptor that mediates tumor cell motility.
    J Cell Biol. 1992 Jun;117(6):1343-50 PMID: 1376732
  71. The mas proto-oncogene is developmentally regulated in the rat central nervous system.
    Brain Res Dev Brain Res. 1992 Jul 24;68(1):75-82 PMID: 1521325
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.125.2.495
Published
1994-04-00
Pages
495-509
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120027
Subset
IM
Grants
NEI NIH HHS · EY06451 · United States
NEI NIH HHS · EY06511 · United States
Databases
GENBANK
Z28366, Z28367
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1083/jcb.125.2.495 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2