Home LiteratureArticle Details
PMID: 7514574 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Entrance and survival of Salmonella typhimurium and Yersinia enterocolitica within human B- and T-cell lines.

Infection and immunity ·Vol. 62 ·No. 6 ·1994-06-00 ·Pages 2229-35

Verjans GM, Ringrose JH, van Alphen L, Feltkamp TE, Kusters JG

Abstract

Lymphocytes, located within the Peyer's patches, might be involved in the dissemination of enteropathogenic Salmonella typhimurium and Yersinia enterocolitica bacteria. To test this hypothesis, we have investigated the susceptibility of human B- and T-cell lines to bacterial adhesion and invasion. The two S. typhimurium strains analyzed were highly invasive, while the two Y. enterocolitica (O:8) strains adhered to the B- and T-cell lines but did not enter the cell lines in significant amounts. We hypothesize that the incapability of the Y. enterocolitica (O:8) strains to enter the human B- and T-cell lines is most probably due to the bacterial inability to induce the internalization process upon adhesion to both cell lines. Although immortalized B- and T-cell lines were used in this study, the results presented suggest the possibility that both cell types could play a role in the dissemination of intracellularly residing S. typhimurium in vivo.

MeSH Terms
B-Lymphocytes/microbiology Bacterial Adhesion Cell Line Humans Integrin beta1 Integrins/analysis Salmonella typhimurium/growth & development T-Lymphocytes/microbiology Yersinia enterocolitica/growth & development
Chemicals
Integrin beta1 Integrins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Verjans G M
Department of Ophthalmo-Immunology, The Netherlands Ophthalmic Research Institute, Amsterdam.
Ringrose J H
van Alphen L
Feltkamp T E
Kusters J G
References (44)
44 references, click to expand
  1. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
  2. Effects of multiplicity of infection, bacterial protein synthesis, and growth phase on adhesion to and invasion of human cell lines by Salmonella typhimurium.
    Infect Immun. 1993 Dec;61(12):5013-20 PMID: 8225577
  3. Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis.
    Infect Immun. 1981 Feb;31(2):775-82 PMID: 7216474
  4. Aromatic-dependent Salmonella typhimurium are non-virulent and effective as live vaccines.
    Nature. 1981 May 21;291(5812):238-9 PMID: 7015147
  5. Invasion of Burkitt's lymphoma cell lines by Yersinia enterocolitica.
    Proc Soc Exp Biol Med. 1983 Feb;172(2):243-9 PMID: 6338506
  6. Construction of a mobilizable Yersinia enterocolitica virulence plasmid.
    J Bacteriol. 1983 Aug;155(2):761-7 PMID: 6874644
  7. Structure, distribution, and origin of M cells in Peyer's patches of mouse ileum.
    Gastroenterology. 1984 May;86(5 Pt 1):789-801 PMID: 6706062
  8. The human cell surface glycoprotein complex (gp 120,200) recognized by monoclonal antibody K20 is a component binding to phytohaemagglutinin on T cells.
    Scand J Immunol. 1986 Jan;23(1):109-18 PMID: 3486453
  9. The VLA protein family. Characterization of five distinct cell surface heterodimers each with a common 130,000 molecular weight beta subunit.
    J Biol Chem. 1987 Mar 5;262(7):3300-9 PMID: 3546305
  10. Plasmid-mediated resistance to phagocytosis in Yersinia enterocolitica.
    Infect Immun. 1987 May;55(5):1176-83 PMID: 3570459
  11. Comparison of the ability of enteroinvasive Escherichia coli, Salmonella typhimurium, Yersinia pseudotuberculosis, and Yersinia enterocolitica to enter and replicate within HEp-2 cells.
    Infect Immun. 1987 Jul;55(7):1674-9 PMID: 3298064
  12. Role of p150,95 in adhesion, migration, chemotaxis and phagocytosis of human monocytes.
    Eur J Immunol. 1987 Sep;17(9):1317-22 PMID: 2958296
  13. Evidence for two genetic loci in Yersinia enterocolitica that can promote invasion of epithelial cells.
    Infect Immun. 1988 May;56(5):1242-8 PMID: 2833444
  14. Factors essential for the penetration of mammalian cells by Yersinia.
    Curr Top Microbiol Immunol. 1988;138:15-39 PMID: 3058389
  15. Intracellular survival of wild-type Salmonella typhimurium and macrophage-sensitive mutants in diverse populations of macrophages.
    Infect Immun. 1989 Jan;57(1):1-7 PMID: 2642463
  16. The ail locus is found uniquely in Yersinia enterocolitica serotypes commonly associated with disease.
    Infect Immun. 1989 Jan;57(1):121-31 PMID: 2642465
  17. Comparison of the invasion strategies used by Salmonella cholerae-suis, Shigella flexneri and Yersinia enterocolitica to enter cultured animal cells: endosome acidification is not required for bacterial invasion or intracellular replication.
    Biochimie. 1988 Aug;70(8):1089-99 PMID: 3147700
  18. Determinants for thermoinducible cell binding and plasmid-encoded cellular penetration detected in the absence of the Yersinia pseudotuberculosis invasin protein.
    Infect Immun. 1989 Jul;57(7):1998-2005 PMID: 2543628
  19. Common themes in microbial pathogenicity.
    Microbiol Rev. 1989 Jun;53(2):210-30 PMID: 2569162
  20. Cloning and molecular characterization of genes whose products allow Salmonella typhimurium to penetrate tissue culture cells.
    Proc Natl Acad Sci U S A. 1989 Aug;86(16):6383-7 PMID: 2548211
  21. Pathogenesis and immunity in murine salmonellosis.
    Microbiol Rev. 1989 Dec;53(4):390-409 PMID: 2687679
  22. Salmonella as an intracellular parasite.
    Mol Microbiol. 1989 Dec;3(12):1833-41 PMID: 2695752
  23. Multiple beta 1 chain integrins are receptors for invasin, a protein that promotes bacterial penetration into mammalian cells.
    Cell. 1990 Mar 9;60(5):861-71 PMID: 2311122
  24. Nonpathogenic isolates of Yersinia enterocolitica do not contain functional inv-homologous sequences.
    Infect Immun. 1990 Apr;58(4):1059-64 PMID: 1690684
  25. Pathways for the penetration of enteroinvasive Yersinia into mammalian cells.
    Mol Biol Med. 1990 Feb;7(1):73-82 PMID: 2182969
  26. Specific inhibition of phagosome-lysosome fusion in murine macrophages mediated by Salmonella typhimurium infection.
    FEMS Microbiol Immunol. 1990 May;2(1):35-43 PMID: 2196071
  27. Segregation of MHC class II molecules from MHC class I molecules in the Golgi complex for transport to lysosomal compartments.
    Nature. 1991 Feb 21;349(6311):669-76 PMID: 1847504
  28. Phase variation of the opacity outer membrane protein controls invasion by Neisseria gonorrhoeae into human epithelial cells.
    EMBO J. 1991 Jun;10(6):1307-15 PMID: 1673923
  29. Discrimination between intracellular uptake and surface adhesion of bacterial pathogens.
    Science. 1991 May 17;252(5008):934-8 PMID: 1674624
  30. Functional evidence for three distinct and independently inhibitable adhesion activities mediated by the human integrin VLA-4. Correlation with distinct alpha 4 epitopes.
    J Biol Chem. 1991 Jun 5;266(16):10241-5 PMID: 1709929
  31. Inhibition of macrophage phagosome-lysosome fusion by Salmonella typhimurium.
    Infect Immun. 1991 Jul;59(7):2232-8 PMID: 2050395
  32. A 'safe-site' for Salmonella typhimurium is within splenic cells during the early phase of infection in mice.
    Microb Pathog. 1991 Apr;10(4):297-310 PMID: 1895929
  33. The invasin protein of Yersinia enterocolitica: internalization of invasin-bearing bacteria by eukaryotic cells is associated with reorganization of the cytoskeleton.
    J Cell Biol. 1992 Jan;116(1):197-207 PMID: 1730744
  34. Adhesion to vascular cell adhesion molecule 1 and fibronectin. Comparison of alpha 4 beta 1 (VLA-4) and alpha 4 beta 7 on the human B cell line JY.
    J Biol Chem. 1992 Apr 25;267(12):8366-70 PMID: 1373725
  35. Involvement of the epidermal growth factor receptor in the invasion of cultured mammalian cells by Salmonella typhimurium.
    Nature. 1992 Jun 18;357(6379):588-9 PMID: 1608468
  36. Measles virus transmembrane fusion protein synthesized de novo or presented in immunostimulating complexes is endogenously processed for HLA class I- and class II-restricted cytotoxic T cell recognition.
    J Exp Med. 1992 Jul 1;176(1):119-28 PMID: 1613454
  37. Very late antigen 4-dependent adhesion and costimulation of resting human T cells by the bacterial beta 1 integrin ligand invasin.
    J Exp Med. 1993 Jan 1;177(1):207-12 PMID: 8418202
  38. The interaction of bacteria with mammalian cells.
    Annu Rev Cell Biol. 1992;8:333-63 PMID: 1476803
  39. Signal transduction in the mammalian cell during bacterial attachment and entry.
    Cell. 1993 Jun 4;73(5):903-20 PMID: 8500180
  40. Ruffles induced by Salmonella and other stimuli direct macropinocytosis of bacteria.
    Nature. 1993 Aug 12;364(6438):639-42 PMID: 8350922
  41. The Yersinia pseudotuberculosis adhesin YadA mediates intimate bacterial attachment to and entry into HEp-2 cells.
    Infect Immun. 1993 Sep;61(9):3914-21 PMID: 7689542
  42. The role of phosphorylation in activation of the alpha 6A beta 1 laminin receptor.
    J Biol Chem. 1993 Sep 5;268(25):18427-30 PMID: 8360143
  43. Current perspectives in reactive arthritis.
    Immunol Today. 1993 Aug;14(8):387-91 PMID: 8397777
  44. Gentamicin antibacterial activity in the presence of human polymorphonuclear leukocytes.
    Antimicrob Agents Chemother. 1979 Dec;16(6):743-9 PMID: 533256
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.62.6.2229-2235.1994
Published
1994-06-00
Pages
2229-35
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC186502
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.62.6.2229-2235.1994 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2