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PMID: 7689542 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Yersinia pseudotuberculosis adhesin YadA mediates intimate bacterial attachment to and entry into HEp-2 cells.

Infection and immunity ·Vol. 61 ·No. 9 ·1993-09-00 ·Pages 3914-21

Bliska JB, Copass MC, Falkow S

Abstract

We characterized a bacterium-host cell interaction that is mediated by the Yersinia adhesin YadA. Derivatives of the virulence plasmid pIB1 harboring mutations in yadA, yopE, or yopH or in a low-calcium-response regulatory locus were introduced into a Yersinia pseudotuberculosis YPIII strain defective for Inv. The mutant strains were tested for the capacity to attach to and enter HEp-2 cells and express the cytotoxic activities of YopE and YopH. As previously shown, expression of YadA was necessary for bacterial attachment and Yop activity in the absence of Inv (R. Rosqvist, A. Forsberg, M. Rimpilainen, T. Bergman, and H. Wolf-Watz, Mol. Microbiol. 4:657-667, 1990). In addition, bacterial entry into HEp-2 cells occurred efficiently when YadA was expressed in the absence of YopE and YopH. These results demonstrated that YadA mediates intimate attachment of Y. pseudotuberculosis to HEp-2 cells and that phagocytic uptake of bacteria by this pathway is inhibited by the synergistic activities of YopH and YopE. A role for beta 1 integrins as host cell receptors for this bacterial attachment and entry mechanism was supported by HEp-2 cell adhesion and monoclonal antibody neutralization studies.

MeSH Terms
Adhesins, Bacterial Bacterial Adhesion Bacterial Outer Membrane Proteins/analysis,metabolism Bacterial Proteins/analysis Humans Integrin beta1 Integrins/physiology Plasmids Protein Tyrosine Phosphatases Tumor Cells, Cultured Virulence Yersinia pseudotuberculosis/pathogenicity
Chemicals
Adhesins, Bacterial Bacterial Outer Membrane Proteins Bacterial Proteins Integrin beta1 Integrins YadA protein, Yersinia yopE protein, Yersinia invasin, Yersinia Protein Tyrosine Phosphatases yopH protein, Yersinia
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bliska J B
Department of Microbiology, School of Medicine, State University of New York, Stony Brook 11794.
Copass M C
Falkow S
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.61.9.3914-3921.1993
Published
1993-09-00
Pages
3914-21
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC281094
Subset
IM
Grants
NIAID NIH HHS · AI07851-01 · United States
External Links
PubMed source DOI: 10.1128/iai.61.9.3914-3921.1993 PMC Full Text View journal details
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