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PMID: 7516405 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

gp39-CD40 interactions are essential for germinal center formation and the development of B cell memory.

The Journal of experimental medicine ·Vol. 180 ·No. 1 ·1994-07-01 ·Pages 157-63

Foy TM, Laman JD, Ledbetter JA, Aruffo A, Claassen E, Noelle RJ

Abstract

gp39, the ligand for CD40 expressed on activated CD4+ T helper cells, is required for the generation of antibody responses to T-dependent (TD) antigens. Treatment of mice with anti-gp39 in vivo inhibits both primary and secondary antibody formation to TD, but not T-independent antigens. However, the role of this receptor-ligand pair in the development of germinal centers and the generation of B cell memory is as yet undefined. Using an antibody to gp39, this study examines the in vivo requirement for gp39-CD40 interactions in the induction of germinal center formation, as well as in the generation of B cell memory. Animals were immunized, treated in vivo with anti-gp39, and evaluated using immunohistochemical staining for the presence of splenic germinal centers 9-11 d after immunization. The results demonstrate that the formation of germinal centers was completely inhibited as a result of treatment with anti-gp39. Moreover, adoptive transfer experiments demonstrate that the generation of antigen-specific memory B cells is also inhibited as a consequence of blocking gp39-CD40 interactions. Taken together, the data demonstrate that gp39-CD40 interactions are critical not only for the generation of antibody responses, but also in the development of B cell memory.

MeSH Terms
Animals Antibody Formation Antigens, CD/physiology Antigens, Differentiation, B-Lymphocyte/physiology B-Lymphocytes/immunology CD40 Antigens CD40 Ligand Female Immunologic Memory Membrane Glycoproteins/physiology Mice Mice, Inbred BALB C
Chemicals
Antigens, CD Antigens, Differentiation, B-Lymphocyte CD40 Antigens Membrane Glycoproteins CD40 Ligand
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Foy T M
Department of Microbiology, Dartmouth Medical School, Lebanon, New Hampshire 03756.
Laman J D
Ledbetter J A
Aruffo A
Claassen E
Noelle R J
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.180.1.157
Published
1994-07-01
Pages
157-63
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2191546
Subset
IM
Grants
NIAID NIH HHS · AI-26296 · United States
External Links
PubMed source DOI: 10.1084/jem.180.1.157 PMC Full Text View journal details
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