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PMID: 7526155 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

MID1, a novel Saccharomyces cerevisiae gene encoding a plasma membrane protein, is required for Ca2+ influx and mating.

Molecular and cellular biology ·Vol. 14 ·No. 12 ·1994-12-00 ·Pages 8259-71

Iida H, Nakamura H, Ono T, Okumura MS, Anraku Y

Abstract

By establishing a unique screening method, we have isolated yeast mutants that die only after differentiating into cells with a mating projection, and some of them are also defective in Ca2+ signaling. The mutants were classified into five complementation groups, one of which we studied extensively. This mutation defines a new gene, designated MID1, which encodes an N-glycosylated, integral plasma membrane protein with 548 amino acid residues. The mid1-1 mutant has low Ca2+ uptake activity, loses viability after receiving mating pheromones, and escapes death when incubated with high concentrations of CaCl2. The MID1 gene is nonessential for vegetative growth. The efficiency of mating between MATa mid1-1 and MAT alpha mid1-1 cells is low. These results demonstrate that MID1 is required for Ca2+ influx and mating.

Related Genes
MID1
MeSH Terms
Amino Acid Sequence Base Sequence Calcium/metabolism Fungal Proteins/chemistry,genetics,metabolism Gene Expression Regulation, Fungal Genes, Fungal Ion Channels/chemistry,genetics Mating Factor Membrane Glycoproteins/chemistry,genetics Methylene Blue Molecular Sequence Data Mutagenesis, Insertional Peptides/pharmacology Phenotype RNA, Messenger/genetics Restriction Mapping Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Signal Transduction Solubility Transcription, Genetic
Chemicals
Fungal Proteins Ion Channels MID1 protein, S cerevisiae Membrane Glycoproteins Peptides RNA, Messenger Saccharomyces cerevisiae Proteins Mating Factor Calcium Methylene Blue
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Iida H
Division of Cell Proliferation, National Institute for Basic Biology, Okazakii, Japan.
Nakamura H
Ono T
Okumura M S
Anraku Y
References (52)
52 references, click to expand
  1. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
    Genetics. 1989 May;122(1):19-27 PMID: 2659436
  2. Putting the HO gene to work: practical uses for mating-type switching.
    Methods Enzymol. 1991;194:132-46 PMID: 2005783
  3. Primary structure and functional expression of the cardiac dihydropyridine-sensitive calcium channel.
    Nature. 1989 Jul 20;340(6230):230-3 PMID: 2474130
  4. Nucleotide sequence of AMS1, the structure gene of vacuolar alpha-mannosidase of Saccharomyces cerevisiae.
    Biochem Biophys Res Commun. 1989 Sep 15;163(2):908-15 PMID: 2675832
  5. AG alpha 1 is the structural gene for the Saccharomyces cerevisiae alpha-agglutinin, a cell surface glycoprotein involved in cell-cell interactions during mating.
    Mol Cell Biol. 1989 Aug;9(8):3155-65 PMID: 2677666
  6. Primary structure and functional expression from complementary DNA of the rod photoreceptor cyclic GMP-gated channel.
    Nature. 1989 Dec 14;342(6251):762-6 PMID: 2481236
  7. KAR3, a kinesin-related gene required for yeast nuclear fusion.
    Cell. 1990 Mar 23;60(6):1029-41 PMID: 2138512
  8. Zinc fingers and other metal-binding domains. Elements for interactions between macromolecules.
    J Biol Chem. 1990 Apr 25;265(12):6513-6 PMID: 2108957
  9. A superfamily of ion channels.
    Nature. 1990 Jun 21;345(6277):672 PMID: 1694264
  10. Essential role for induced Ca2+ influx followed by [Ca2+]i rise in maintaining viability of yeast cells late in the mating pheromone response pathway. A study of [Ca2+]i in single Saccharomyces cerevisiae cells with imaging of fura-2.
    J Biol Chem. 1990 Aug 5;265(22):13391-9 PMID: 2198292
  11. Yeast Saccharomyces cerevisiae selectable markers in pUC18 polylinkers.
    Yeast. 1990 Sep-Oct;6(5):363-6 PMID: 2220072
  12. Basic local alignment search tool.
    J Mol Biol. 1990 Oct 5;215(3):403-10 PMID: 2231712
  13. Cell cycle control by Ca2+ in Saccharomyces cerevisiae.
    J Biol Chem. 1990 Dec 5;265(34):21216-22 PMID: 2123488
  14. Synchronization of haploid yeast cell cycles, a prelude to conjugation.
    Exp Cell Res. 1973 Jan;76(1):111-7 PMID: 4566309
  15. Reversible arrest of haploid yeast cells in the initiation of DNA synthesis by a diffusible sex factor.
    Exp Cell Res. 1973 Jan;76(1):99-110 PMID: 4566314
  16. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  17. Cell-cell recognition in Saccharomyces cerevisiae: regulation of mating-specific adhesion.
    J Bacteriol. 1978 Jun;134(3):893-901 PMID: 350854
  18. Isolation of yeast histone genes H2A and H2B.
    Cell. 1979 Dec;18(4):1261-71 PMID: 519767
  19. Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast.
    Methods Enzymol. 1991;194:281-301 PMID: 2005793
  20. Positional mapping of genes by chromosome blotting and chromosome fragmentation.
    Methods Enzymol. 1991;194:57-77 PMID: 2005810
  21. Genetic screens and selections for cell and nuclear fusion mutants.
    Methods Enzymol. 1991;194:774-92 PMID: 2005824
  22. Cell cycle control by calcium and calmodulin in Saccharomyces cerevisiae.
    Biochim Biophys Acta. 1991 Jul 10;1093(2-3):169-77 PMID: 1863597
  23. Yeast has homologs (CNA1 and CNA2 gene products) of mammalian calcineurin, a calmodulin-regulated phosphoprotein phosphatase.
    Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7376-80 PMID: 1651503
  24. A putative zinc finger protein, Saccharomyces cerevisiae Vps18p, affects late Golgi functions required for vacuolar protein sorting and efficient alpha-factor prohormone maturation.
    Mol Cell Biol. 1991 Dec;11(12):5813-24 PMID: 1840635
  25. Signal transduction during pheromone response in yeast.
    Annu Rev Cell Biol. 1991;7:699-728 PMID: 1667085
  26. Regulatory subunit (CNB1 gene product) of yeast Ca2+/calmodulin-dependent phosphoprotein phosphatases is required for adaptation to pheromone.
    Mol Cell Biol. 1992 Aug;12(8):3460-9 PMID: 1321337
  27. Characterization of the energy-dependent, mating factor-activated Ca2+ influx in Saccharomyces cerevisiae.
    Cell Calcium. 1992 Nov;13(10):615-26 PMID: 1337499
  28. The a-factor transporter (STE6 gene product) and cell polarity in the yeast Saccharomyces cerevisiae.
    J Cell Biol. 1993 Mar;120(5):1203-15 PMID: 7679674
  29. Epitope mapping and accessibility of immunodominant regions of yeast plasma membrane H(+)-ATPase.
    Eur J Biochem. 1993 Mar 15;212(3):737-44 PMID: 7681777
  30. MKK1 and MKK2, which encode Saccharomyces cerevisiae mitogen-activated protein kinase-kinase homologs, function in the pathway mediated by protein kinase C.
    Mol Cell Biol. 1993 May;13(5):3076-83 PMID: 8386320
  31. Protein phosphatase type 2B (calcineurin)-mediated, FK506-sensitive regulation of intracellular ions in yeast is an important determinant for adaptation to high salt stress conditions.
    EMBO J. 1993 Nov;12(11):4063-71 PMID: 7693452
  32. Molecular cloning and expression of the Saccharomyces cerevisiae RFC3 gene, an essential component of replication factor C.
    Proc Natl Acad Sci U S A. 1994 Feb 1;91(3):868-72 PMID: 8302859
  33. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  34. High efficiency transformation of Escherichia coli with plasmids.
    Gene. 1990 Nov 30;96(1):23-8 PMID: 2265755
  35. Isolation and genetic analysis of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones.
    Mol Cell Biol. 1982 Jan;2(1):11-20 PMID: 7050665
  36. Transformation of protoplasted yeast cells is directly associated with cell fusion.
    Mol Cell Biol. 1984 Apr;4(4):771-8 PMID: 6371497
  37. The structure of an antigenic determinant in a protein.
    Cell. 1984 Jul;37(3):767-78 PMID: 6204768
  38. Specific induction of Ca2+ transport activity in MATa cells of Saccharomyces cerevisiae by a mating pheromone, alpha factor.
    J Biol Chem. 1985 Sep 5;260(19):10482-6 PMID: 3161880
  39. A rapid, efficient method for isolating DNA from yeast.
    Gene. 1986;42(2):169-73 PMID: 3015730
  40. KAR1, a gene required for function of both intranuclear and extranuclear microtubules in yeast.
    Cell. 1987 Mar 27;48(6):1047-60 PMID: 3030557
  41. Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein.
    Mol Cell Biol. 1987 Jul;7(7):2316-28 PMID: 3302672
  42. Identification and regulation of a gene required for cell fusion during mating of the yeast Saccharomyces cerevisiae.
    Mol Cell Biol. 1987 Aug;7(8):2680-90 PMID: 3313002
  43. The Saccharomyces cerevisiae BAR1 gene encodes an exported protein with homology to pepsin.
    Proc Natl Acad Sci U S A. 1988 Jan;85(1):55-9 PMID: 3124102
  44. Primary structure of rat brain sodium channel III deduced from the cDNA sequence.
    FEBS Lett. 1988 Feb 8;228(1):187-94 PMID: 2449363
  45. Conjugation in Saccharomyces cerevisiae.
    Annu Rev Cell Biol. 1988;4:429-57 PMID: 2848554
  46. Life cycle of the budding yeast Saccharomyces cerevisiae.
    Microbiol Rev. 1988 Dec;52(4):536-53 PMID: 3070323
  47. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites.
    Gene. 1988 Dec 30;74(2):527-34 PMID: 3073106
  48. Lyticase: endoglucanase and protease activities that act together in yeast cell lysis.
    J Bacteriol. 1980 May;142(2):414-23 PMID: 6991473
  49. Control of yeast cell type by the mating type locus. I. Identification and control of expression of the a-specific gene BAR1.
    J Mol Biol. 1981 Dec 5;153(2):305-21 PMID: 7040681
  50. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  51. BIK1, a protein required for microtubule function during mating and mitosis in Saccharomyces cerevisiae, colocalizes with tubulin.
    J Cell Biol. 1990 Dec;111(6 Pt 1):2573-86 PMID: 2277073
  52. KAR2, a karyogamy gene, is the yeast homolog of the mammalian BiP/GRP78 gene.
    Cell. 1989 Jun 30;57(7):1211-21 PMID: 2661018
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.12.8259-8271.1994
Published
1994-12-00
Pages
8259-71
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359365
Subset
IM
Databases
GENBANK
D32133
External Links
PubMed source DOI: 10.1128/mcb.14.12.8259-8271.1994 PMC Full Text View journal details
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