Abstract
In this report we describe the isolation and characterization of a monoclonal antibody against human serum transferrin (Tf) and the cloning and sequencing of its cDNA. The antibody competes with the transferrin receptor (TR) for binding to human Tf and is therefore expected to bind at or very close to a region of interaction between Tf and its receptor. From the deduced amino acid sequence, we constructed a 3-dimensional model of the variable domains of the antibody based on the canonical structure model for the hypervariable loops. The proposed structure of the antibody is a first step toward a more detailed characterization of the antibody-Tf complex and possibly toward a better understanding of the Tf interaction with its receptor. The model might prove useful in guiding site-directed mutagenesis studies, simplifying the experimental elucidation of the antibody structure, and in the use of automatic procedures to dock the interacting molecules as soon as structural information about the structure of the human Tf molecule will be available.
MeSH Terms
Amino Acid Sequence
Animals
Antibodies, Monoclonal/genetics,pharmacology
Base Sequence
Binding Sites, Antibody
Binding, Competitive
Cells, Cultured
Epitopes
Female
Humans
Immunoglobulin Heavy Chains/genetics
Immunoglobulin Light Chains/genetics
Immunoglobulin Variable Region/genetics
Mice
Mice, Inbred BALB C
Models, Molecular
Molecular Sequence Data
Receptors, Transferrin/drug effects
Transferrin/immunology
Chemicals
Antibodies, Monoclonal
Epitopes
Immunoglobulin Heavy Chains
Immunoglobulin Light Chains
Immunoglobulin Variable Region
Receptors, Transferrin
Transferrin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Orlandini M
Department of Molecular Biology, University of Siena, Italy.
Santucci A
Tramontano A
Neri P
Oliviero S
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