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PMID: 7559348 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning, nucleotide sequence, and regulation of katE encoding a sigma B-dependent catalase in Bacillus subtilis.

Journal of bacteriology ·Vol. 177 ·No. 19 ·1995-10-00 ·Pages 5598-605

Engelmann S, Lindner C, Hecker M

Abstract

A sigma B-dependent stress gene of Bacillus subtilis was localized downstream of the licS gene. The predicted amino acid sequence exhibited a significant similarity to the sequence of the katE-encoded catalase HPII of Escherichia coli, and we designated it the open reading frame katE. In a B. subtilis katE mutant, catalase 2 could not be detected. The amount of katE-specific mRNA was increased after heat, salt, or ethanol stress or after glucose starvation in a sigma B-dependent manner. As in E. coli, the transcription of the katE gene in B. subtilis was unaffected by the addition of H2O2 to exponentially growing cells. In contrast, the katA gene encoding catalase 1 of B. subtilis showed an induction pattern different from that of katE; katA expression was strongly increased by oxidative stress. The similarity between E. coli sigma S-dependent genes and B. subtilis sigma B-dependent genes suggests that both may confer multiple stress resistance to stationary-phase cells.

MeSH Terms
Amino Acid Sequence Bacillus subtilis/enzymology,genetics Bacterial Proteins/physiology Base Sequence Catalase/biosynthesis,chemistry,genetics Cloning, Molecular Enzyme Induction/drug effects Ethanol/pharmacology Gene Expression Regulation, Bacterial/genetics Genes, Bacterial/genetics Glucose/metabolism Heat-Shock Response Molecular Sequence Data Oxidative Stress Promoter Regions, Genetic/genetics RNA, Bacterial/biosynthesis RNA, Messenger/biosynthesis Sequence Analysis, DNA Sequence Homology, Amino Acid Sigma Factor/physiology Sodium Chloride/pharmacology Transcription Factors/physiology Transcription, Genetic/genetics
Chemicals
Bacterial Proteins RNA, Bacterial RNA, Messenger SigB protein, Bacteria Sigma Factor Transcription Factors Ethanol Sodium Chloride Catalase Glucose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Engelmann S
Institut für Mikrobiologie und Molekularbiologie, Ernst-Moritz-Arndt-Universität, Greifswald, Germany.
Lindner C
Hecker M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.19.5598-5605.1995
Published
1995-10-00
Pages
5598-605
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177370
Subset
IM
Databases
GENBANK
D45911, X85182
External Links
PubMed source DOI: 10.1128/jb.177.19.5598-5605.1995 PMC Full Text View journal details
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