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PMID: 7559351 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Catabolite inactivation of the yeast maltose transporter occurs in the vacuole after internalization by endocytosis.

Journal of bacteriology ·Vol. 177 ·No. 19 ·1995-10-00 ·Pages 5622-7

Riballo E, Herweijer M, Wolf DH, Lagunas R

Abstract

The maltose transporter of Saccharomyces cerevisiae is rapidly degraded during fermentation in the absence of a nitrogen source. The location and mechanism of degradation of the transporter have been investigated. Using mutants defective in endocytosis, we have shown that degradation of this transporter requires internalization by endocytosis. In addition, studies of mutants defective in proteasome or vacuolar proteolysis revealed that degradation occurs in the vacuole and is independent of proteasome function. The results also revealed that degradation of the maltose transporter requires Sec18p and raised the question of whether in the absence of Sec18p activity the internalized maltose transporter is recycled back to the plasma membrane.

MeSH Terms
Adenosine Triphosphatases Biological Transport, Active Carrier Proteins/metabolism Cysteine Endopeptidases/physiology Endocytosis Fungal Proteins/genetics,metabolism,physiology Maltose/metabolism Monosaccharide Transport Proteins Multienzyme Complexes/physiology Mutation Proteasome Endopeptidase Complex Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins Symporters Temperature Vacuoles/metabolism Vesicular Transport Proteins
Chemicals
Carrier Proteins Fungal Proteins Monosaccharide Transport Proteins Multienzyme Complexes Saccharomyces cerevisiae Proteins Symporters Vesicular Transport Proteins maltose transport system, S cerevisiae Maltose Cysteine Endopeptidases Proteasome Endopeptidase Complex Adenosine Triphosphatases SEC18 protein, S cerevisiae
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Riballo E
Instituto de Investigaciones Biomédicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Herweijer M
Wolf D H
Lagunas R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.19.5622-5627.1995
Published
1995-10-00
Pages
5622-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177373
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.177.19.5622-5627.1995 PMC Full Text View journal details
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