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PMID: 7559788 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A postprophase topoisomerase II-dependent chromatid core separation step in the formation of metaphase chromosomes.

The Journal of cell biology ·Vol. 131 ·No. 1 ·1995-10-00 ·Pages 7-17

Giménez-Abián JF, Clarke DJ, Mullinger AM, Downes CS, Johnson RT

Abstract

Metaphase chromatids are believed to consist of loops of chromatin anchored to a central scaffold, of which a major component is the decatenatory enzyme DNA topoisomerase II. Silver impregnation selectively stains an axial element of metaphase and anaphase chromatids; but we find that in earlier stages of mitosis, silver staining reveals an initially single, folded midline structure, which separates at prometaphase to form two chromatid axes. Inhibition of topoisomerase II prevents this separation, and also prevents the contraction of chromatids that occurs when metaphase is arrested. Immunolocalization of topoisomerase II alpha reveals chromatid cores analogous to those seen with silver staining. We conclude that the chromatid cores in early mitosis form a single structure, constrained by DNA catenations, which must separate before metaphase chromatids can be resolved.

MeSH Terms
Animals Cell Line, Transformed/cytology,enzymology Chromatids/enzymology Chromosome Aberrations/chemically induced Chromosome Disorders Chromosomes/enzymology DNA Topoisomerases, Type II/analysis,metabolism Diketopiperazines Enzyme Inhibitors/pharmacology Fibroblasts/cytology,enzymology Metaphase/physiology Mitosis/physiology Muntjacs Piperazines/pharmacology Prophase/physiology Silver Staining Time Factors Topoisomerase II Inhibitors
Chemicals
Diketopiperazines Enzyme Inhibitors Piperazines Topoisomerase II Inhibitors 4,4'-(1,2-dimethyl-1,2-ethanediyl)bis-2,6-piperazinedione DNA Topoisomerases, Type II
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Giménez-Abián J F
Department of Zoology, University of Cambridge, United Kingdom.
Clarke D J
Mullinger A M
Downes C S
Johnson R T
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.131.1.7
Published
1995-10-00
Pages
7-17
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120606
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.131.1.7 PMC Full Text View journal details
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