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PMID: 7565675 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The DNA-binding properties of two heat shock factors, HSF1 and HSF3, are induced in the avian erythroblast cell line HD6.

Molecular and cellular biology ·Vol. 15 ·No. 10 ·1995-10-00 ·Pages 5268-78

Nakai A, Kawazoe Y, Tanabe M, Nagata K, Morimoto RI

Abstract

Avian cells express three heat shock transcription factor (HSF) genes corresponding to a novel factor, HSF3, and homologs of mouse and human HSF1 and HSF2. Analysis of the biochemical and cell biological properties of these HSFs reveals that HSF3 has properties in common with both HSF1 and HSF2 and yet has features which are distinct from both. HSF3 is constitutively expressed in the erythroblast cell line HD6, the lymphoblast cell line MSB, and embryo fibroblasts, and yet its DNA-binding activity is induced only upon exposure of HD6 cells to heat shock. Acquisition of HSF3 DNA-binding activity in HD6 cells is accompanied by oligomerization from a non-DNA-binding dimer to a DNA-binding trimer, whereas the effect of heat shock on HSF1 is oligomerization of an inert monomer to a DNA-binding trimer. Induction of HSF3 DNA-binding activity is delayed compared with that of HSF1. As occurs for HSF1, heat shock leads to the translocation of HSF3 to the nucleus. HSF exhibits the properties of a transcriptional activator, as judged from the stimulatory activity of transiently overexpressed HSF3 measured by using a heat shock element-containing reporter construct and as independently assayed by the activity of a chimeric GAL4-HSF3 protein on a GAL4 reporter construct. These results reveal that HSF3 is negatively regulated in avian cells and acquires DNA-binding activity in certain cells upon heat shock.

MeSH Terms
Animals Avian Proteins Base Sequence Cell Line Cell Nucleus/metabolism Chick Embryo Chickens DNA/metabolism DNA-Binding Proteins/biosynthesis,chemistry,metabolism Erythroblasts/metabolism Fibroblasts HSP70 Heat-Shock Proteins/genetics Heat Shock Transcription Factors Heat-Shock Proteins/metabolism Hot Temperature Humans Mice Molecular Sequence Data Molecular Weight Promoter Regions, Genetic/genetics Protein Conformation Quail T-Lymphocytes Trans-Activators/biosynthesis,chemistry,metabolism Transcription Factors/metabolism
Chemicals
Avian Proteins DNA-Binding Proteins HSF1 protein, human HSF3 protein, Gallus gallus HSP70 Heat-Shock Proteins Heat Shock Transcription Factors Heat-Shock Proteins Trans-Activators Transcription Factors HSF2 protein, human DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Nakai A
Department of Cell Biology, Kyoto University, Japan.
Kawazoe Y
Tanabe M
Nagata K
Morimoto R I
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.10.5268
Published
1995-10-00
Pages
5268-78
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230774
Subset
IM
Grants
NIGMS NIH HHS · GM38109 · United States
External Links
PubMed source DOI: 10.1128/MCB.15.10.5268 PMC Full Text View journal details
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