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PMID: 7760831 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A heat shock-responsive domain of human HSF1 that regulates transcription activation domain function.

Molecular and cellular biology ·Vol. 15 ·No. 6 ·1995-06-00 ·Pages 3354-62

Green M, Schuetz TJ, Sullivan EK, Kingston RE

Abstract

Human heat shock factor 1 (HSF1) stimulates transcription from heat shock protein genes following stress. We have used chimeric proteins containing the GAL4 DNA binding domain to identify the transcriptional activation domains of HSF1 and a separate domain that is capable of regulating activation domain function. This regulatory domain conferred heat shock inducibility to chimeric proteins containing the activation domains. The regulatory domain is located between the transcriptional activation domains and the DNA binding domain of HSF1 and is conserved between mammalian and chicken HSF1 but is not found in HSF2 or HSF3. The regulatory domain was found to be functionally homologous between chicken and human HSF1. This domain does not affect DNA binding by the chimeric proteins and does not contain any of the sequences previously postulated to regulate DNA binding of HSF1. Thus, we suggest that activation of HSF1 by stress in humans is controlled by two regulatory mechanisms that separately confer heat shock-induced DNA binding and transcriptional stimulation.

MeSH Terms
Amino Acid Sequence DNA-Binding Proteins/chemistry,genetics,metabolism Gene Expression Regulation HeLa Cells Heat Shock Transcription Factors Hot Temperature Humans Molecular Sequence Data Peptide Mapping Transcription Factors Transcriptional Activation/genetics
Chemicals
DNA-Binding Proteins HSF1 protein, human Heat Shock Transcription Factors Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Green M
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114, USA.
Schuetz T J
Sullivan E K
Kingston R E
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36 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.6.3354
Published
1995-06-00
Pages
3354-62
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230569
Subset
IM
Grants
NIGMS NIH HHS · GM43901 · United States
External Links
PubMed source DOI: 10.1128/MCB.15.6.3354 PMC Full Text View journal details
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