Home LiteratureArticle Details
PMID: 7565701 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The zinc finger transcription factor Egr-1 potentiates macrophage differentiation of hematopoietic cells.

Molecular and cellular biology ·Vol. 15 ·No. 10 ·1995-10-00 ·Pages 5499-507

Krishnaraju K, Nguyen HQ, Liebermann DA, Hoffman B

Abstract

Previously we have shown that the zinc finger transcription factor Egr-1 is essential for and restricts differentiation of hematopoietic cells along the macrophage lineage, raising the possibility that Egr-1 actually plays a deterministic role in governing the development of hematopoietic precursor cells along the monocytic lineage. To test this hypothesis, we have taken advantage of interleukin-3-dependent 32Dcl3 hematopoietic precursor cells which, in addition to undergoing granulocytic differentiation in response to granulocyte colony-stimulating factor, were found to be induced for limited proliferation, but not differentiation, by granulocyte-macrophage colony-stimulating factor. It was shown that ectopic expression of Egr-1 blocked granulocyte colony-stimulating factor-induced terminal granulocytic differentiation, consistent with previous findings. In addition, ectopic expression of Egr-1 endowed 32Dcl3 cells with ability to be induced by granulocyte-macrophage colony-stimulating factor for terminal differentiation exclusively along the macrophage lineage. Thus, evidence that Egr-1 potentiates terminal macrophage differentiation has been obtained, suggesting that Egr-1 plays a deterministic role in governing the development of hematopoietic cells along the macrophage lineage.

MeSH Terms
Animals Cell Differentiation Cell Division Cell Line DNA-Binding Proteins/biosynthesis,physiology Early Growth Response Protein 1 Gene Expression/drug effects Granulocyte Colony-Stimulating Factor/pharmacology Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology Granulocytes/cytology Hematopoiesis/drug effects Hematopoietic Stem Cells/cytology Immediate-Early Proteins Interleukin-3/pharmacology Macrophages/cytology Mice RNA, Messenger/analysis Transcription Factors/biosynthesis,physiology Zinc Fingers
Chemicals
DNA-Binding Proteins Early Growth Response Protein 1 Egr1 protein, mouse Immediate-Early Proteins Interleukin-3 RNA, Messenger Transcription Factors Granulocyte Colony-Stimulating Factor Granulocyte-Macrophage Colony-Stimulating Factor
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Krishnaraju K
Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.
Nguyen H Q
Liebermann D A
Hoffman B
References (29)
29 references, click to expand
  1. Control of normal differentiation of myeloid leukemic cells. XI. Induction of a specific requirement for cell viability and growth during the differentiation of myeloid leukemic cells.
    J Cell Physiol. 1976 Oct;89(2):259-66 PMID: 972167
  2. Regulation and role of different macrophage-and granulocyte-inducing proteins in normal and leukemic myeloid cells.
    Int J Cancer. 1982 Feb 15;29(2):159-61 PMID: 6977501
  3. Constitutive synthesis of interleukin-3 by leukaemia cell line WEHI-3B is due to retroviral insertion near the gene.
    Nature. 1985 Sep 19-25;317(6034):255-8 PMID: 2413359
  4. Expression of a set of growth-related immediate early genes in BALB/c 3T3 cells: coordinate regulation with c-fos or c-myc.
    Proc Natl Acad Sci U S A. 1987 Mar;84(5):1182-6 PMID: 3469660
  5. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  6. A nerve growth factor-induced gene encodes a possible transcriptional regulatory factor.
    Science. 1987 Nov 6;238(4828):797-9 PMID: 3672127
  7. The molecular control of blood cell development.
    Science. 1987 Dec 4;238(4832):1374-9 PMID: 3317831
  8. A novel early growth response gene rapidly induced by fibroblast, epithelial cell and lymphocyte mitogens.
    Oncogene Res. 1987 Sep-Oct;1(4):343-55 PMID: 3130602
  9. Two mouse genes encoding potential transcription factors with identical DNA-binding domains are activated by growth factors in cultured cells.
    Proc Natl Acad Sci U S A. 1988 Jul;85(13):4691-5 PMID: 3133658
  10. A gene activated in mouse 3T3 cells by serum growth factors encodes a protein with "zinc finger" sequences.
    Proc Natl Acad Sci U S A. 1988 Nov;85(21):7857-61 PMID: 3141919
  11. The molecular control of cell division, differentiation commitment and maturation in haemopoietic cells.
    Nature. 1989 May 4;339(6219):27-30 PMID: 2469962
  12. Proto-oncogene expression and dissection of the myeloid growth to differentiation developmental cascade.
    Oncogene. 1989 May;4(5):583-92 PMID: 2471131
  13. Granulocyte-macrophage colony-stimulating factor and tetradecanoyl phorbol acetate induce a distinct, restricted subset of primary-response TIS genes in both proliferating and terminally differentiated myeloid cells.
    Mol Cell Biol. 1989 Aug;9(8):3580-3 PMID: 2677677
  14. Complexity of the immediate early response of myeloid cells to terminal differentiation and growth arrest includes ICAM-1, Jun-B and histone variants.
    Oncogene. 1990 Mar;5(3):387-96 PMID: 1690380
  15. Induction of the granulocyte-macrophage colony-stimulating factor (CSF) receptor by granulocyte CSF increases the differentiative options of a murine hematopoietic progenitor cell.
    Mol Cell Biol. 1990 Sep;10(9):4846-53 PMID: 1697033
  16. Methylation of an immediate-early inducible gene as a mechanism for B cell tolerance induction.
    Science. 1990 Nov 9;250(4982):797-800 PMID: 2237429
  17. Dissection of the immediate early response of myeloid leukemia cells to terminal differentiation and growth inhibitory stimuli.
    Cell Growth Differ. 1990 Dec;1(12):637-45 PMID: 2126951
  18. Interleukin-6- and leukemia inhibitory factor-induced terminal differentiation of myeloid leukemia cells is blocked at an intermediate stage by constitutive c-myc.
    Mol Cell Biol. 1991 May;11(5):2375-81 PMID: 1901940
  19. Retinoic acid increases zif268 early gene expression in rat preosteoblastic cells.
    Mol Cell Biol. 1991 May;11(5):2503-10 PMID: 1708092
  20. Posttranscriptional regulation of the zinc finger-encoding EGR-1 gene by granulocyte-macrophage colony-stimulating factor in human U-937 monocytic leukemia cells: involvement of a pertussis toxin-sensitive G protein.
    Cell Growth Differ. 1991 Jun;2(6):273-8 PMID: 2064996
  21. In vivo and in vitro effects of TGF-beta 1 on normal and neoplastic haemopoiesis.
    Ciba Found Symp. 1991;157:212-27; discussion 227-31 PMID: 1649034
  22. Interferon regulatory factor 1 is a myeloid differentiation primary response gene induced by interleukin 6 and leukemia inhibitory factor: role in growth inhibition.
    Cell Growth Differ. 1991 Aug;2(8):401-7 PMID: 1793735
  23. Cloning of the low-affinity murine granulocyte-macrophage colony-stimulating factor receptor and reconstitution of a high-affinity receptor complex.
    Proc Natl Acad Sci U S A. 1992 May 15;89(10):4295-9 PMID: 1533931
  24. Deregulated c-myb disrupts interleukin-6- or leukemia inhibitory factor-induced myeloid differentiation prior to c-myc: role in leukemogenesis.
    Mol Cell Biol. 1992 Jun;12(6):2493-500 PMID: 1588953
  25. Proto-oncogenes of the fos/jun family of transcription factors are positive regulators of myeloid differentiation.
    Mol Cell Biol. 1993 Feb;13(2):841-51 PMID: 8423806
  26. The zinc finger transcription factor Egr-1 is essential for and restricts differentiation along the macrophage lineage.
    Cell. 1993 Jan 29;72(2):197-209 PMID: 7678779
  27. Cytogenetic and molecular delineation of the smallest commonly deleted region of chromosome 5 in malignant myeloid diseases.
    Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5484-8 PMID: 8516290
  28. The novel primary response gene MyD118 and the proto-oncogenes myb, myc, and bcl-2 modulate transforming growth factor beta 1-induced apoptosis of myeloid leukemia cells.
    Mol Cell Biol. 1994 Apr;14(4):2352-60 PMID: 8139540
  29. Growth and differentiation proceeds normally in cells deficient in the immediate early gene NGFI-A.
    J Biol Chem. 1995 Apr 28;270(17):9971-7 PMID: 7730380
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.10.5499
Published
1995-10-00
Pages
5499-507
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230800
Subset
IM
Grants
NCI NIH HHS · 1RO1CA43618 · United States
NCI NIH HHS · 1RO1CA51162 · United States
NCI NIH HHS · 1RO1CA59774 · United States
External Links
PubMed source DOI: 10.1128/MCB.15.10.5499 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2