Abstract
Bacillus cereus causes exotoxin-mediated diarrheal food poisoning. Hemolysin BL (HBL) is a well-characterized B. cereus toxin composed of three components (B, L1, and L2) that together possess hemolytic, cytotoxic, dermonecrotic, and vascular permeability activities. Here, we show that HBL causes fluid accumulation in ligated rabbit ileal loops at a dose of 5 micrograms of each component per loop. Maximal fluid responses occurred for combinations of all three components at > or = 25 micrograms of each component per loop. Individual components and binary combinations did not cause significant fluid accumulation at 25 micrograms of each component. Specific antisera to HBL components inhibited the fluid accumulation response of crude culture supernatant from B. cereus F837/76. These antisera were tested against an antiserum to a partially characterized multicomponent diarrheal toxin described previously by Thompson et al. (N. E. Thompson, M. J. Ketterhagen, M. S. Bergdoll, and E. J. Shantz, Infect. Immun. 43:887-894, 1984). Immunoblot and immunoprecipitation analyses indicate that HBL and that toxin are identical. These results confirm previous speculation that HBL is a tripartite enterotoxin that, as for all of its other known activities, requires all three components for maximal activity.
MeSH Terms
Animals
Antibodies, Bacterial/immunology
Bacillus cereus/pathogenicity
Bacterial Proteins/immunology,toxicity
Bacterial Toxins/toxicity
Diarrhea/microbiology
Enterotoxins/immunology,toxicity
Female
Hemolysin Proteins/toxicity
Ileum
Immunodiffusion
Rabbits
Chemicals
Antibodies, Bacterial
Bacterial Proteins
Bacterial Toxins
Enterotoxins
Hemolysin Proteins
hemolysin BL protein, Bacillus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Beecher D J
Department of Food Microbiology and Toxicology, University of Wisconsin-Madison, USA.
Schoeni J L
Wong A C
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