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PMID: 7592413 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The physical map of the chromosome of a serogroup A strain of Neisseria meningitidis shows complex rearrangements relative to the chromosomes of the two mapped strains of the closely related species N. gonorrhoeae.

Journal of bacteriology ·Vol. 177 ·No. 22 ·1995-11-00 ·Pages 6390-400

Dempsey JA, Wallace AB, Cannon JG

Abstract

A physical map of the chromosome of N. meningitidis Z2491 (serogroup A, subgroup IV-1) has been constructed. Z2491 DNA was digested with NheI, SpeI, SgfI, PacI, BglII, or PmeI, resulting in a limited number of fragments that were resolved by contour-clamped homogeneous electric field (CHEF) electrophoresis. The estimated genome size for this strain was 2,226 kb. To construct the map, probes corresponding to single-copy genes or sequences were used on Southern blots of chromosomal DNA digested with the different mapping enzymes and subjected to CHEF electrophoresis. By determining which fragments from different digests hybridized to each specific probe, it was possible to walk back and forth between digests to form a circular macrorestriction map. The intervals between mapped restriction sites range from 10 to 143 kb in size. A total of 117 markers have been placed on the map; 75 represent identified genes, with the remaining markers defined by anonymous cloned fragments of neisserial DNA. Comparison of the arrangement of genetic loci in Z2491 with that in gonococcal strain FA1090, for which a physical map was previously constructed, revealed complex genomic rearrangements between the two strains. Although gene order is generally conserved over much of the chromosome, a region of approximately 500 kb shows translocation and/or inversion of multiple blocks of markers between the two strains. Even within the relatively conserved portions of the maps, several genetic markers are in different positions in Z2491 and FA1090.

MeSH Terms
Base Sequence Chromosome Inversion Chromosome Walking/methods Chromosomes, Bacterial DNA Probes Deoxyribonucleases, Type II Site-Specific Electrophoresis, Gel, Pulsed-Field Gene Rearrangement Genetic Markers Molecular Sequence Data Neisseria gonorrhoeae/genetics Neisseria meningitidis/genetics Restriction Mapping Translocation, Genetic
Chemicals
DNA Probes Genetic Markers Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dempsey J A
Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill 27599, USA.
Wallace A B
Cannon J G
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.22.6390-6400.1995
Published
1995-11-00
Pages
6390-400
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177488
Subset
IM
Grants
NIAID NIH HHS · AI23830 · United States
NIAID NIH HHS · AI28807 · United States
External Links
PubMed source DOI: 10.1128/jb.177.22.6390-6400.1995 PMC Full Text View journal details
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