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PMID: 7592498 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Delineation of AbrB-binding sites on the Bacillus subtilis spo0H, kinB, ftsAZ, and pbpE promoters and use of a derived homology to identify a previously unsuspected binding site in the bsuB1 methylase promote.

Journal of bacteriology ·Vol. 177 ·No. 23 ·1995-12-00 ·Pages 6999-7002

Strauch MA

Abstract

DNase I footprinting experiments showed that AbrB binds to the regulatory regions of the spo0H, kinB, ftsAZ, and pbpE genes. A conserved motif was found in these and other AbrB-binding sites. A search for Bacillus subtilis DNA sequences containing this motif led to the prediction that AbrB would bind to the promoter controlling the bsuB1 methylase gene. DNase I footprinting experiments confirmed this prediction.

MeSH Terms

Bacillus subtilis/genetics Bacterial Proteins/metabolism Base Sequence Binding Sites/genetics Consensus Sequence DNA Footprinting DNA-Binding Proteins/metabolism Genes, Bacterial/genetics Molecular Sequence Data Promoter Regions, Genetic Sequence Homology, Nucleic Acid Site-Specific DNA-Methyltransferase (Adenine-Specific)/genetics Transcription Factors/metabolism

Chemicals

AbrB protein, Bacillus subtilis Bacterial Proteins DNA-Binding Proteins Transcription Factors DNA modification methylase PstI Site-Specific DNA-Methyltransferase (Adenine-Specific)

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Strauch M A

Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037, USA.

References (20)

20 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.177.23.6999-7002.1995

Published

1995-12-00

Pages

6999-7002

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC177573

Subset

Grants

NIGMS NIH HHS · GM46700 · United States

External Links

PubMed source DOI: 10.1128/jb.177.23.6999-7002.1995 PMC Full Text View journal details

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Delineation of AbrB-binding sites on the Bacillus subtilis spo0H, kinB, ftsAZ, and pbpE promoters and use of a derived homology to identify a previously unsuspected binding site in the bsuB1 methylase promote.

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