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PMID: 7593171 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Rapid, widespread, and longlasting induction of nestin contributes to the generation of glial scar tissue after CNS injury.

The Journal of cell biology ·Vol. 131 ·No. 2 ·1995-10-00 ·Pages 453-64

Frisén J, Johansson CB, Török C, Risling M, Lendahl U

Abstract

Neuronal regeneration does generally not occur in the central nervous system (CNS) after injury, which has been attributed to the generation of glial scar tissue. In this report we show that the composition of the glial scar after traumatic CNS injury in rat and mouse is more complex than previously assumed: expression of the intermediate filament nestin is induced in reactive astrocytes. Nestin induction occurs within 48 hours in the spinal cord both at the site of lesion and in degenerating tracts and lasts for at least 13 months. Nestin expression is induced with similar kinetics in the crushed optic nerve. In addition to the expression in reactive astrocytes, we also observed nestin induction within 48 hours after injury in cells close to the central canal in the spinal cord, while nestin expressing cells at later timepoints were found progressively further out from the central canal. This dynamic pattern of nestin induction after injury was mimicked by lacZ expressing cells in nestin promoter/lacZ transgenic mice, suggesting that defined nestin regulatory regions mediate the injury response. We discuss the possibility that the spatiotemporal pattern of nestin expression reflects a population of nestin positive cells, which proliferates and migrates from a region close to the central canal to the site of lesion in response to injury.

MeSH Terms
Animals Astrocytes/metabolism,pathology Biomarkers Cell Count Cells, Cultured Central Nervous System/injuries,metabolism Cicatrix/metabolism Fluorescent Antibody Technique, Indirect Intermediate Filament Proteins/analysis,biosynthesis Male Mice Mice, Inbred C57BL Nerve Tissue Proteins Nestin Rats Rats, Wistar Wound Healing
Chemicals
Biomarkers Intermediate Filament Proteins Nerve Tissue Proteins Nes protein, mouse Nes protein, rat Nestin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Frisén J
Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
Johansson C B
Török C
Risling M
Lendahl U
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.131.2.453
Published
1995-10-00
Pages
453-64
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2199991
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.131.2.453 PMC Full Text View journal details
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