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PMID: 8464882 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Vimentin mRNA location changes during muscle development.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 7 ·1993-04-01 ·Pages 2724-8

Cripe L, Morris E, Fulton AB

Abstract

The mRNAs for some cytoskeletal proteins are localized, suggesting that mRNA for these proteins may concentrate at sites appropriate for assembly. To test this hypothesis, we observed vimentin mRNA in developing chicken muscle cultures by in situ hybridization with a digoxigenin-labeled DNA probe to vimentin, detected by confocal microscopy using fluorescent anti-digoxigenin antibody. This method has submicrometer resolution. In developing muscle, vimentin mRNA was bipolar in young myoblasts, somewhat perinuclear in elongated myoblasts and spread fibroblasts, and diffuse in young and developing myotubes. In mature myotubes, vimentin mRNA occurred at costameres with vimentin protein. Localization of mRNA may prove important for assembling and maintaining differentiated cytoskeletal structures, as it is for organizing the embryo.

MeSH Terms

Animals Antibodies, Monoclonal Base Sequence Cells, Cultured Chick Embryo Codon DNA/genetics Molecular Sequence Data Muscles/cytology,embryology,physiology Oligodeoxyribonucleotides Oligonucleotide Probes Plasmids Polymerase Chain Reaction/methods RNA, Messenger/analysis,genetics,metabolism Vimentin/analysis,biosynthesis,genetics

Chemicals

Antibodies, Monoclonal Codon Oligodeoxyribonucleotides Oligonucleotide Probes RNA, Messenger Vimentin DNA

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Cripe L

Department of Biochemistry, University of Iowa, Iowa City 52242.

Morris E

Fulton A B

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24 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.90.7.2724

Published

1993-04-01

Pages

2724-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC46168

Subset

External Links

PubMed source DOI: 10.1073/pnas.90.7.2724 PMC Full Text View journal details

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Vimentin mRNA location changes during muscle development.

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