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PMID: 7597098 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Shk1, a homolog of the Saccharomyces cerevisiae Ste20 and mammalian p65PAK protein kinases, is a component of a Ras/Cdc42 signaling module in the fission yeast Schizosaccharomyces pombe.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 13 ·1995-06-20 ·Pages 6180-4

Marcus S, Polverino A, Chang E, Robbins D, Cobb MH, Wigler MH

Abstract

We describe a protein kinase, Shk1, from the fission yeast Schizosaccharomyces pombe, which is structurally related to the Saccharomyces cerevisiae Ste20 and mammalian p65PAK protein kinases. We provide genetic evidence for physical and functional interaction between Shk1 and the Cdc42 GTP-binding protein required for normal cell morphology and mating in S. pombe. We further show that expression of the STE20 gene complements the shk1 null mutation and that Shk1 is capable of signaling to the pheromone-responsive mitogen-activated protein kinase cascade in S. cerevisiae. Our results lead us to propose that signaling modules composed of small GTP-binding proteins and protein kinases related to Shk1, Ste20, and p65PAK, are highly conserved in evolution and participate in both cytoskeletal functions and mitogen-activated protein kinase signaling pathways.

Related Genes
STE20 cdc42 shk1
MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Cycle Proteins/metabolism Crosses, Genetic DNA Primers Fungal Proteins/metabolism GTP-Binding Proteins/metabolism Gene Deletion Genes, Fungal Intracellular Signaling Peptides and Proteins MAP Kinase Kinase Kinases Mammals Molecular Sequence Data Protein Serine-Threonine Kinases/biosynthesis,genetics,metabolism Regulatory Sequences, Nucleic Acid Saccharomyces cerevisiae/enzymology Saccharomyces cerevisiae Proteins Schizosaccharomyces/enzymology,genetics,physiology Schizosaccharomyces pombe Proteins Sequence Deletion Sequence Homology, Amino Acid Signal Transduction beta-Galactosidase/analysis,biosynthesis cdc42 GTP-Binding Protein p21-Activated Kinases ras Proteins
Chemicals
Cell Cycle Proteins DNA Primers Fungal Proteins Intracellular Signaling Peptides and Proteins Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins Protein Serine-Threonine Kinases p21-Activated Kinases shk1 protein, S pombe MAP Kinase Kinase Kinases STE20 protein, S cerevisiae beta-Galactosidase GTP-Binding Proteins cdc42 GTP-Binding Protein ras Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Marcus S
Cold Spring Harbor Laboratory, NY 11724, USA.
Polverino A
Chang E
Robbins D
Cobb M H
Wigler M H
References (33)
33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.13.6180
Published
1995-06-20
Pages
6180-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC41666
Subset
IM
Databases
GENBANK
L41552
External Links
PubMed source DOI: 10.1073/pnas.92.13.6180 PMC Full Text View journal details
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