Home LiteratureArticle Details
PMID: 7608098 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Regulation of the multiple antibiotic resistance (mar) regulon by marORA sequences in Escherichia coli.

Journal of bacteriology ·Vol. 177 ·No. 14 ·1995-07-00 ·Pages 4176-8

Martin RG, Nyantakyi PS, Rosner JL

Abstract

The mar operon and adjacent sequences were subcloned on a low-copy-number plasmid to identify essential regulatory elements. A 1.1-kbp fragment containing 133 bp of the operator-promoter region (marO), the full marRA gene sequences, and only 10 of 72 marB codons provided a dela mar strain with normal repressibility and inducibility and the ability to beget mar constitutive mutants.

Related Genes
marA marO marR
MeSH Terms
Bacterial Proteins/genetics DNA Mutational Analysis DNA-Binding Proteins Drug Resistance, Microbial/genetics Drug Resistance, Multiple/genetics Escherichia coli/genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial Mutagenesis Operator Regions, Genetic/genetics Promoter Regions, Genetic/genetics Regulon/genetics Repressor Proteins/genetics Sequence Deletion Transcription Factors/genetics
Chemicals
Bacterial Proteins DNA-Binding Proteins Escherichia coli Proteins MarA protein, E coli MarR protein, E coli Repressor Proteins Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Martin R G
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892-0560, USA.
Nyantakyi P S
Rosner J L
References (15)
15 references, click to expand
  1. Gene in the major cotransduction gap of the Escherichia coli K-12 linkage map required for the expression of chromosomal resistance to tetracycline and other antibiotics.
    J Bacteriol. 1983 Aug;155(2):541-8 PMID: 6307967
  2. Binding of purified multiple antibiotic-resistance repressor protein (MarR) to mar operator sequences.
    Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5456-60 PMID: 7777530
  3. Mu d-directed lacZ fusions regulated by low pH in Escherichia coli.
    J Bacteriol. 1987 Jul;169(7):3001-6 PMID: 2954947
  4. Characterization and in vivo cloning of prlC, a suppressor of signal sequence mutations in Escherichia coli K12.
    Genetics. 1987 Aug;116(4):513-21 PMID: 2957272
  5. marA locus causes decreased expression of OmpF porin in multiple-antibiotic-resistant (Mar) mutants of Escherichia coli.
    J Bacteriol. 1988 Dec;170(12):5416-22 PMID: 2848006
  6. Activation of oxidative stress genes by mutations at the soxQ/cfxB/marA locus of Escherichia coli.
    J Bacteriol. 1991 Jul;173(14):4433-9 PMID: 1648558
  7. marA, a regulated locus which controls expression of chromosomal multiple antibiotic resistance in Escherichia coli.
    J Bacteriol. 1991 Sep;173(17):5532-8 PMID: 1715857
  8. pH dependence and gene structure of inaA in Escherichia coli.
    J Bacteriol. 1992 Mar;174(5):1537-43 PMID: 1537798
  9. Genetic and functional analysis of the multiple antibiotic resistance (mar) locus in Escherichia coli.
    J Bacteriol. 1993 Mar;175(5):1484-92 PMID: 8383113
  10. Overexpression of the MarA positive regulator is sufficient to confer multiple antibiotic resistance in Escherichia coli.
    J Bacteriol. 1993 May;175(10):2888-94 PMID: 8491710
  11. Salicylate induction of antibiotic resistance in Escherichia coli: activation of the mar operon and a mar-independent pathway.
    J Bacteriol. 1993 Dec;175(24):7856-62 PMID: 7504664
  12. Repressor mutations in the marRAB operon that activate oxidative stress genes and multiple antibiotic resistance in Escherichia coli.
    J Bacteriol. 1994 Jan;176(1):143-8 PMID: 8282690
  13. Dual regulation of inaA by the multiple antibiotic resistance (mar) and superoxide (soxRS) stress response systems of Escherichia coli.
    J Bacteriol. 1994 Oct;176(20):6262-9 PMID: 7928997
  14. Analysis of the genetic requirements for inducible multiple-antibiotic resistance associated with the mar locus in Escherichia coli.
    J Bacteriol. 1994 Dec;176(24):7754-6 PMID: 8002604
  15. P1 plasmid replication. Role of initiator titration in copy number control.
    J Mol Biol. 1986 Nov 20;192(2):275-85 PMID: 3560217
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.14.4176-4178.1995
Published
1995-07-00
Pages
4176-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177157
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.177.14.4176-4178.1995 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2