Home LiteratureArticle Details
PMID: 8002604 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis of the genetic requirements for inducible multiple-antibiotic resistance associated with the mar locus in Escherichia coli.

Journal of bacteriology ·Vol. 176 ·No. 24 ·1994-12-00 ·Pages 7754-6

Sulavik MC, Gambino LF, Miller PF

Abstract

A series of novel genetic constructs derived from the marRAB operon was used to determine the role of this gene cluster in salicylate-inducible multiple-antibiotic resistance in Escherichia coli. Our findings indicate that regulated antibiotic resistance associated with this locus requires only the products of marR and marA, without any neighboring genes.

Related Genes
marA marB marR
MeSH Terms
Bacterial Proteins/genetics DNA-Binding Proteins Drug Resistance, Microbial Drug Resistance, Multiple/genetics Escherichia coli/drug effects,genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial Mutagenesis Operon/genetics Repressor Proteins/genetics
Chemicals
Bacterial Proteins DNA-Binding Proteins Escherichia coli Proteins MarA protein, E coli MarR protein, E coli Repressor Proteins multiple antibiotic resistance protein B
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sulavik M C
Therapeutics Department, Parke-Davis Pharmaceutical Research Division of Warner-Lambert Co., Ann Arbor, Michigan 48106-1047.
Gambino L F
Miller P F
References (18)
18 references, click to expand
  1. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.
    J Mol Biol. 1976 Jul 5;104(3):541-55 PMID: 781293
  2. Genetic relationship between soxRS and mar loci in promoting multiple antibiotic resistance in Escherichia coli.
    Antimicrob Agents Chemother. 1994 Aug;38(8):1773-9 PMID: 7986007
  3. Transposable elements in prokaryotes.
    Annu Rev Genet. 1981;15:341-404 PMID: 6279020
  4. Gene in the major cotransduction gap of the Escherichia coli K-12 linkage map required for the expression of chromosomal resistance to tetracycline and other antibiotics.
    J Bacteriol. 1983 Aug;155(2):541-8 PMID: 6307967
  5. A pSC101-derived plasmid which shows no sequence homology to other commonly used cloning vectors.
    Gene. 1984 Nov;31(1-3):165-71 PMID: 6098521
  6. Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli.
    J Bacteriol. 1985 Mar;161(3):1219-21 PMID: 2982787
  7. Nonheritable resistance to chloramphenicol and other antibiotics induced by salicylates and other chemotactic repellents in Escherichia coli K-12.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8771-4 PMID: 3909154
  8. The terminus region of the Escherichia coli chromosome contains two separate loci that exhibit polar inhibition of replication.
    Proc Natl Acad Sci U S A. 1987 Apr;84(7):1754-8 PMID: 3550796
  9. Improved single and multicopy lac-based cloning vectors for protein and operon fusions.
    Gene. 1987;53(1):85-96 PMID: 3596251
  10. Positive control of a global antioxidant defense regulon activated by superoxide-generating agents in Escherichia coli.
    Proc Natl Acad Sci U S A. 1990 Aug;87(16):6181-5 PMID: 1696718
  11. Activation of oxidative stress genes by mutations at the soxQ/cfxB/marA locus of Escherichia coli.
    J Bacteriol. 1991 Jul;173(14):4433-9 PMID: 1648558
  12. marA, a regulated locus which controls expression of chromosomal multiple antibiotic resistance in Escherichia coli.
    J Bacteriol. 1991 Sep;173(17):5532-8 PMID: 1715857
  13. Uses of transposons with emphasis on Tn10.
    Methods Enzymol. 1991;204:139-80 PMID: 1658561
  14. Genetic and functional analysis of the multiple antibiotic resistance (mar) locus in Escherichia coli.
    J Bacteriol. 1993 Mar;175(5):1484-92 PMID: 8383113
  15. Overexpression of the MarA positive regulator is sufficient to confer multiple antibiotic resistance in Escherichia coli.
    J Bacteriol. 1993 May;175(10):2888-94 PMID: 8491710
  16. Salicylate induction of antibiotic resistance in Escherichia coli: activation of the mar operon and a mar-independent pathway.
    J Bacteriol. 1993 Dec;175(24):7856-62 PMID: 7504664
  17. Repressor mutations in the marRAB operon that activate oxidative stress genes and multiple antibiotic resistance in Escherichia coli.
    J Bacteriol. 1994 Jan;176(1):143-8 PMID: 8282690
  18. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.24.7754-7756.1994
Published
1994-12-00
Pages
7754-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC197238
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.176.24.7754-7756.1994 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2