Home LiteratureArticle Details
PMID: 7609088 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Suppression of simian immunodeficiency virus replication by human immunodeficiency virus type 1 trans-dominant negative rev mutants.

Journal of virology ·Vol. 69 ·No. 8 ·1995-08-00 ·Pages 5164-6

Endres CL, Bergquam EP, Axthelm MK, Wong SW

Abstract

We demonstrate that trans-dominant negative rev mutants are able to suppress simian immunodeficiency virus provirus replication in both transient cotransfection assays and stably transduced HUT 78 cells. These studies suggest that the efficacy of trans-dominant rev strategies in reducing viral burden may be evaluated in a simian immunodeficiency virus-rhesus macaque animal model.

Related Genes
rev
MeSH Terms
Amino Acid Sequence Cell Line Genes, Dominant Genes, rev Genetic Vectors HIV-1/genetics Humans Molecular Sequence Data Mutation Simian Immunodeficiency Virus/physiology Transduction, Genetic Virus Replication/genetics
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Endres C L
Division of Pathobiology and Immunology, Oregon Regional Primate Research Center, Beaverton 97006, USA.
Bergquam E P
Axthelm M K
Wong S W
References (21)
21 references, click to expand
  1. Inhibition of human immunodeficiency virus type 1 in human T cells by a potent Rev response element decoy consisting of the 13-nucleotide minimal Rev-binding domain.
    J Virol. 1994 Dec;68(12):8254-64 PMID: 7966618
  2. Expression of the neomycin-resistance (neo) gene induces alterations in gene expression and metabolism.
    Hum Gene Ther. 1994 Apr;5(4):449-56 PMID: 7914094
  3. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  4. Characterization of the AIDS-associated retrovirus reverse transcriptase and optimal conditions for its detection in virions.
    Virology. 1985 Dec;147(2):326-35 PMID: 2416116
  5. Use of eukaryotic expression technology in the functional analysis of cloned genes.
    Methods Enzymol. 1987;152:684-704 PMID: 3657593
  6. Gene therapy. Intracellular immunization.
    Nature. 1988 Sep 29;335(6189):395-6 PMID: 3166513
  7. Simian immunodeficiency viruses.
    Annu Rev Microbiol. 1988;42:607-25 PMID: 2849374
  8. Functional dissection of the HIV-1 Rev trans-activator--derivation of a trans-dominant repressor of Rev function.
    Cell. 1989 Jul 14;58(1):205-14 PMID: 2752419
  9. Improved retroviral vectors for gene transfer and expression.
    Biotechniques. 1989 Oct;7(9):980-2, 984-6, 989-90 PMID: 2631796
  10. Induction of AIDS in rhesus monkeys by molecularly cloned simian immunodeficiency virus.
    Science. 1990 Jun 1;248(4959):1109-12 PMID: 2160735
  11. The simian immunodeficiency viruses.
    Annu Rev Immunol. 1990;8:557-78 PMID: 2188674
  12. Mutants in a conserved region near the carboxy-terminus of HIV-1 Rev identify functionally important residues and exhibit a dominant negative phenotype.
    Virology. 1990 Sep;178(1):327-30 PMID: 2202148
  13. Construction and properties of retrovirus packaging cells based on gibbon ape leukemia virus.
    J Virol. 1991 May;65(5):2220-4 PMID: 1850008
  14. Mutational definition of the human immunodeficiency virus type 1 Rev activation domain.
    J Virol. 1991 Aug;65(8):4248-54 PMID: 2072452
  15. Stable expression of transdominant Rev protein in human T cells inhibits human immunodeficiency virus replication.
    J Exp Med. 1992 Oct 1;176(4):1197-201 PMID: 1402661
  16. Inhibition of human immunodeficiency virus type 1 replication in human T cells by retroviral-mediated gene transfer of a dominant-negative Rev trans-activator.
    Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9870-4 PMID: 1409715
  17. In vitro differentiation of monocytoid THP-1 cells affects their permissiveness for HIV strains: a model system for studying the cellular basis of HIV differential tropism.
    Virology. 1993 Mar;193(1):256-67 PMID: 8438570
  18. Long-term noncytopathic productive infection of the human monocytic leukemia cell line THP-1 by human immunodeficiency virus type 1 (HIV-1IIIB).
    Virology. 1993 Apr;193(2):877-87 PMID: 8460491
  19. High-efficiency gene transfer to primary monkey airway epithelial cells with retrovirus vectors using the gibbon ape leukemia virus receptor.
    Hum Gene Ther. 1993 Apr;4(2):161-70 PMID: 8494925
  20. Potent inhibition of human immunodeficiency virus type 1 replication by an intracellular anti-Rev single-chain antibody.
    Proc Natl Acad Sci U S A. 1994 May 24;91(11):5075-9 PMID: 8197188
  21. Molecular and virological effects of intracellular anti-Rev single-chain variable fragments on the expression of various human immunodeficiency virus-1 strains.
    Hum Gene Ther. 1994 Nov;5(11):1315-24 PMID: 7893803
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.69.8.5164-5166.1995
Published
1995-08-00
Pages
5164-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC189339
Subset
IM
Grants
NCRR NIH HHS · RR00163 · United States
External Links
PubMed source DOI: 10.1128/JVI.69.8.5164-5166.1995 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2