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PMID: 8197188 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Retracted Publication

Potent inhibition of human immunodeficiency virus type 1 replication by an intracellular anti-Rev single-chain antibody.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 11 ·1994-05-24 ·Pages 5075-9

Duan L, Bagasra O, Laughlin MA, Oakes JW, Pomerantz RJ

Abstract

Human immunodeficiency virus type 1 (HIV-1) has a complex life cycle, which has made it a difficult target for conventional therapeutic modalities. A single-chain antibody moiety, directed against the HIV-1 regulatory protein Rev, which rescues unspliced viral RNA from the nucleus of infected cells, has now been developed. This anti-Rev single-chain construct (SFv) consists of both light and heavy chain variable regions of anti-Rev monoclonal antibody, which, when expressed intracellularly within human cells, potently inhibits HIV-1 replication. This intracellular SFv molecule is demonstrated to specifically antagonize Rev function. Thus, intracellular SFv expression, against a retroviral regulatory protein, may be useful as a gene therapeutic approach to combat HIV-1 infections.

MeSH Terms

Amino Acid Sequence Animals Antiviral Agents Blotting, Northern Fluorescent Antibody Technique Gene Products, rev/immunology HIV Antibodies/genetics,immunology HIV-1/immunology HeLa Cells Humans Mice Molecular Sequence Data Transfection Virus Replication rev Gene Products, Human Immunodeficiency Virus

Chemicals

Antiviral Agents Gene Products, rev HIV Antibodies rev Gene Products, Human Immunodeficiency Virus

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Duan L

Dorrance H. Hamilton Laboratories, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107.

Bagasra O

Laughlin M A

Oakes J W

Pomerantz R J

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19 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.91.11.5075

Published

1994-05-24

Pages

5075-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC43934

Subset

Grants

NIAID NIH HHS · AI31836 · United States

Corrections

CommentIn

PMID: 12458562

RetractionIn

PMID: 12465612

CommentIn

PMID: 12469684

External Links

PubMed source DOI: 10.1073/pnas.91.11.5075 PMC Full Text View journal details

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Potent inhibition of human immunodeficiency virus type 1 replication by an intracellular anti-Rev single-chain antibody.

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