Characterisation of plasmids coding for the restriction endonuclease EcoRI.

Molecular & general genetics : MGG ·Vol. 143 ·No. 3 ·1976-02-02 ·Pages 319-25

Smith HR, Humphreys GO, Willshaw GA, Anderson ES

Abstract

The properties of two plasmids coding for the CcoRI restriction and modification enzymes are described. Both plasmids are non auto-transferring (NTP) but can be mobilised by transfer factors. Strains carrying NTP13 produce colicin E1 and the EcoRI enzymes. This plasmid has a molecular weight of 6 X 10(6) daltons and is present as approximately 12 copies per chromosome. The second plasmid, NTP14, was detected after mobilisation of the EcoRI plasmid with the R factor RI-19. NTP14 codes for ampicillin resistance, synthesis of the EcoRI enzymes and colicin E1. The molecular weight of NTP14 is 10.7 X 10(6) daltons and there are about 14 copies per chromosome. DNA-DNA reassociation experiments were performed to determine the interrelationships of NTP13, NTP14, ColE1 and the R factor R1-19. NTP13 and NTP14 continue to replicate when cellular protein synthesis is inhibited by the addition of chloramphenicol.

MeSH Terms

Ampicillin/pharmacology Chloramphenicol/pharmacology DNA, Bacterial/isolation & purification Endonucleases/biosynthesis Escherichia coli/enzymology Extrachromosomal Inheritance Genetic Code Lysogeny Penicillin Resistance Plasmids Streptomycin/pharmacology Sulfonamides/pharmacology Tetracyclines/pharmacology

Chemicals

DNA, Bacterial Sulfonamides Tetracyclines Chloramphenicol Ampicillin Endonucleases Streptomycin

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Smith H R

Humphreys G O

Willshaw G A

Anderson E S

References (30)

30 references, click to expand

R factor-controlled restriction and modification of deoxyribonucleic acid: restriction mutants.
J Bacteriol. 1972 Dec;112(3):1275-9 PMID: 4565538
Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
DNA--protein complexes of delta-mediated transfer systems.
Biochim Biophys Acta. 1972 Dec 6;287(2):355-60 PMID: 4609474
Molecular studies of R factor compatibility groups.
J Bacteriol. 1973 Jul;115(1):387-98 PMID: 4577745
A simple method for the preparation of large quantities of pure plasmid DNA.
Biochim Biophys Acta. 1975 Apr 2;383(4):457-63 PMID: 1092355
Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
J Bacteriol. 1972 May;110(2):667-76 PMID: 4336693
Properties of an R factor from Pseudomonas aeruginosa.
J Bacteriol. 1971 Dec;108(3):1244-9 PMID: 4945193
Repressor-minus and operator-constitutive de-repressed mutants of F-like R factors: their effect on chromosomal transfer by HfrC.
Genet Res. 1969 Dec;14(3):309-13 PMID: 4904464
Genetic and molecular characterisation of some non-transferring plasmids.
Mol Gen Genet. 1974 Mar 27;129(3):229-42 PMID: 4601266
Circular DNA forms of colicinogenic factors E1, E2 and E3 from Escherichia coli.
J Mol Biol. 1968 Sep 14;36(2):185-94 PMID: 4939624
[Studies on the frequency of transductive properties of colicinogenic strains].
C R Seances Soc Biol Fil. 1956 Sep 26;150(5):1036-9 PMID: 13375022
I-like resistance factors with the fi+ character.
Genet Res. 1971 Jun;17(3):267-71 PMID: 4946932
Characterization of a transfer factor associated with drug resistance in Salmonella typhimurium.
Nature. 1965 Nov 27;208(5013):843-9 PMID: 5331112
Study of the association of resistance to two drugs in a transferable determinant in Salmonella typhimurium.
Genet Res. 1968 Feb;11(1):119-24 PMID: 4869959
DNA restriction and modification mechanisms in bacteria.
Annu Rev Microbiol. 1971;25:153-76 PMID: 4949033
Transduction of resistance determinants and R factors of the transfer systems by phage Plkc.
Mol Gen Genet. 1972;114(3):261-5 PMID: 4552505
Filamentous phages specific for the I sex factor.
Nature. 1968 Mar 23;217(5134):1184-6 PMID: 4868371
Transposition of ampicillin resistance from RP4 to other replicons.
Mol Gen Genet. 1974;132(1):31-40 PMID: 4609125
Staphylococcal penicillinase. I. Inhibition and stimulation of activity.
J Bacteriol. 1961 Aug;82:298-304 PMID: 13747000
Origin of the TEM-beta-lactamase gene found on plasmids.
J Bacteriol. 1975 Apr;122(1):250-6 PMID: 1091625
Molecular weights of coliphages and coliphage DNA. 3. Contour length and molecular weight of DNA from bacteriophages T4, T5 and T7, and from bovine papilloma virus.
J Mol Biol. 1970 Dec 28;54(3):557-65 PMID: 4321637
The ecology of transferable drug resistance in the enterobacteria.
Annu Rev Microbiol. 1968;22:131-80 PMID: 4879515
Drug resistance and its transfer in Salmonella typhimurium.
Nature. 1965 May 8;206(984):579-83 PMID: 5319285
Colicins.
Annu Rev Microbiol. 1957;11:7-22 PMID: 13470810
The molecular nature and replication of drug resistance factors of the Enterobacteriaceae.
Ann N Y Acad Sci. 1971 Jun 11;182:188-206 PMID: 4936662
R factor compatibility groups.
Mol Gen Genet. 1972;119(4):287-97 PMID: 4567804
A new phage-typing scheme for Salmonella typhi-murium.
J Hyg (Lond). 1959 Sep;57:346-59 PMID: 13807011
The effects of Salmonella typhimurium on derepressed mutants of F-like factors.
Genet Res. 1971 Feb;17(1):89-93 PMID: 4933323
R124, an fi R factor of a new compatibility class.
J Gen Microbiol. 1972 Jul;71(2):403-5 PMID: 4558791
DNA modification and restriction.
Prog Nucleic Acid Res Mol Biol. 1974;14(0):1-37 PMID: 4602489

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00269410

Published

1976-02-02

Pages

319-25

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00269410

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Characterisation of plasmids coding for the restriction endonuclease EcoRI.

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