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PMID: 7678588 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mycobacterium avium-M. intracellulare binds to the integrin receptor alpha v beta 3 on human monocytes and monocyte-derived macrophages.

Infection and immunity ·Vol. 61 ·No. 2 ·1993-02-00 ·Pages 663-70

Rao SP, Ogata K, Catanzaro A

Abstract

Mycobacterium avium-M. intracellulare is an intracellular pathogen responsible for the highest incidence of disseminated bacterial infection in patients with AIDS. Treatment of the infection is difficult and has been of limited efficacy. Attachment of the organism to macrophages is a critical early step in the establishment of the disease. In the present study, we isolated and identified a receptor that mediates attachment of M. avium-M. intracellulare to human peripheral blood monocytes and monocyte-derived macrophages. On Western blotting, (immunoblotting), the receptor was found to cross-react with antibodies against a human vitronectin receptor (alpha v beta 3). The receptor could be purified from monocyte extracts by using monoclonal antibodies (MAbs) against the alpha v subunit of vitronectin receptor coupled to CNBr-Sepharose 4B, as well as with the adhesive tripeptide sequence arginine-glycine-aspartic acid (RGD) coupled to CNBr-Sepharose 4B. Surface-bound MAbs directed against alpha v beta 3 were found to inhibit the attachment of M. avium-M. intracellulare to monocyte-derived macrophages in an in vitro inhibition assay, while MAbs directed against CD14, CD18, alpha 2 beta 1 and platelet glycoprotein gpIIb/IIIa receptors did not inhibit this attachment. These observations suggest that alpha v beta 3 on the surface of human monocytes and monocyte-derived macrophages may function as a receptor for M. avium-M. intracellulare. Identification of a receptor for M. avium-M. intracellulare on macrophages may offer new approaches to the prevention and control of M. avium-M. intracellulare infection at the cellular level.

MeSH Terms
Amino Acid Sequence Antibodies, Monoclonal/immunology Bacterial Adhesion Cells, Cultured Chromatography, Affinity Cross Reactions Humans Integrins/analysis,immunology,isolation & purification Macrophages/microbiology Molecular Sequence Data Molecular Weight Monocytes/microbiology Mycobacterium avium Complex/pathogenicity,physiology Oligopeptides/metabolism Receptors, Cytoadhesin/analysis,immunology,isolation & purification Receptors, Vitronectin
Chemicals
Antibodies, Monoclonal Integrins Oligopeptides Receptors, Cytoadhesin Receptors, Vitronectin arginyl-glycyl-aspartic acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rao S P
Department of Medicine, University of California, San Diego 92103-8374.
Ogata K
Catanzaro A
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.61.2.663-670.1993
Published
1993-02-00
Pages
663-70
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC302778
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.61.2.663-670.1993 PMC Full Text View journal details
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