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PMID: 7678980 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of the proto-oncogenes c-met and c-kit and their ligands, hepatocyte growth factor/scatter factor and stem cell factor, in SCLC cell lines and xenografts.

British journal of cancer ·Vol. 67 ·No. 1 ·1993-01-00 ·Pages 37-46

Rygaard K, Nakamura T, Spang-Thomsen M

Abstract

We examined a panel of 25 small cell lung cancer (SCLC) cell lines and nude mouse xenografts for expression of the proto-oncogenes c-met and c-kit, and for expression of the corresponding ligands, hepatocyte growth factor (HGF) (also known as scatter factor (SF)), and stem cell factor (SCF), respectively. Expression of mRNA was detected by Northern blotting, and c-met and c-kit protein expression was detected by Western blotting and immunocytochemistry. c-met and c-kit mRNA was expressed in 22 of the examined cell lines or xenografts, and coexpression of the two proto-oncogenes was observed in 20 tumours. Expression of c-met and c-kit protein paralleled in the mRNA expression. HGF/SF mRNA was expressed in two of the examined tumours, and only one of these also expressed the c-met proto-oncogene. SCF mRNA was expressed in 19 of the examined tumours, and in 18 of these coexpression of c-kit and SCF was present. The high percentage of SCLC tumours expressing c-met and c-kit indicates that these proto-oncogenes may have an important function in this disease. The rare coexpression of c-met and HGF/SF is evidence that an autocrine regulatory pathway is not present for this receptor/ligand system in SCLC, while the frequent coexpression of c-kit and SCF indicates that this receptor/ligand system may have an autocrine function in SCLC.

MeSH Terms
Amino Acid Sequence Animals Blotting, Western Carcinoma, Small Cell/genetics Gene Expression/genetics Hematopoietic Cell Growth Factors/genetics,physiology Hepatocyte Growth Factor/genetics,physiology Humans Immunohistochemistry Lung Neoplasms/genetics Mice Mice, Inbred BALB C Mice, Nude Molecular Sequence Data Proto-Oncogene Mas Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-kit Proto-Oncogene Proteins c-met Proto-Oncogenes/genetics RNA, Messenger/genetics Stem Cell Factor Transcription, Genetic Transplantation, Heterologous Tumor Cells, Cultured
Chemicals
Hematopoietic Cell Growth Factors MAS1 protein, human Proto-Oncogene Mas Proto-Oncogene Proteins RNA, Messenger Stem Cell Factor Hepatocyte Growth Factor Proto-Oncogene Proteins c-kit Proto-Oncogene Proteins c-met
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rygaard K
University Institute of Pathological Anatomy, Copenhagen, Denmark.
Nakamura T
Spang-Thomsen M
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Article Info
Journal
British journal of cancer
Abbr.
Br J Cancer
ISSN
0007-0920
DOI
10.1038/bjc.1993.7
Published
1993-01-00
Pages
37-46
Language
English
Region
England
NLM ID
0370635
PMCID
PMC1968226
Subset
IM
External Links
PubMed source DOI: 10.1038/bjc.1993.7 PMC Full Text View journal details
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