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PMID: 7679508 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

MEF2C, a MADS/MEF2-family transcription factor expressed in a laminar distribution in cerebral cortex.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 4 ·1993-02-15 ·Pages 1546-50

Leifer D, Krainc D, Yu YT, McDermott J, Breitbart RE, Heng J, Neve RL, Kosofsky B, Nadal-Ginard B, Lipton SA

Abstract

We have cloned cDNA encoding a human transcription factor that belongs to the MEF2 (myocyte-specific enhancer-binding factor 2) subfamily of the MADS (MCM1-agamous-deficiens-serum response factor) gene family. This factor, which we have named MEF2C, binds specifically to the MEF2 element and activates transcription via this element. Specific isoforms of this factor are found exclusively in brain and are robustly expressed by neurons in cerebral cortex. In situ hybridization indicates that the factor is expressed preferentially in certain neuronal layers of cortex and that expression declines during postnatal development. The unusual pattern of expression in brain suggests that this transcription factor may be important in the development of cortical architecture.

MeSH Terms
Amino Acid Sequence Animals Blotting, Northern Brain/physiology Cerebral Cortex/physiology Chloramphenicol O-Acetyltransferase/genetics,metabolism Cloning, Molecular DNA/genetics,metabolism DNA-Binding Proteins/genetics Fetus Gene Library Genetic Vectors Humans MADS Domain Proteins MEF2 Transcription Factors Molecular Sequence Data Multigene Family Myogenic Regulatory Factors Organ Specificity RNA/genetics,isolation & purification Rats Recombinant Fusion Proteins/metabolism Sequence Homology, Amino Acid Transcription Factors/genetics Transfection beta-Galactosidase/genetics,metabolism
Chemicals
DNA-Binding Proteins MADS Domain Proteins MEF2 Transcription Factors MEF2C protein, human Myogenic Regulatory Factors Recombinant Fusion Proteins Transcription Factors RNA DNA Chloramphenicol O-Acetyltransferase beta-Galactosidase
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Leifer D
Department of Neurology, Children's Hospital, Boston, MA.
Krainc D
Yu Y T
McDermott J
Breitbart R E
Heng J
Neve R L
Kosofsky B
Nadal-Ginard B
Lipton S A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.4.1546
Published
1993-02-15
Pages
1546-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC45911
Subset
IM
Grants
NEI NIH HHS · EY06087 · United States
NICHD NIH HHS · HD00888 · United States
Databases
GENBANK
L08895
External Links
PubMed source DOI: 10.1073/pnas.90.4.1546 PMC Full Text View journal details
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