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PMID: 7680654 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cortactin, an 80/85-kilodalton pp60src substrate, is a filamentous actin-binding protein enriched in the cell cortex.

The Journal of cell biology ·Vol. 120 ·No. 6 ·1993-03-00 ·Pages 1417-26

Wu H, Parsons JT

Abstract

Two related cellular proteins, p80 and p85 (cortactin), become phosphorylated on tyrosine in pp60src-transformed cells and in cells stimulated with certain growth factors. The amino-terminal half of cortactin is comprised of multiple copies of an internal, tandem 37-amino acid repeat. The carboxyl-terminal half contains a distal SH3 domain. We report that cortactin is an F-actin-binding protein. The binding to F-actin is specific and saturable. The amino-terminal repeat region appears to be both necessary and sufficient to mediate actin binding, whereas the SH3 domain had no apparent effect on the actin-binding activity. Cortactin, present in several different cell types, is enriched in cortical structures such as membrane ruffles and lamellipodia. The properties of cortactin indicate that it may be important for microfilament-membrane interactions as well as transducing signals from the cell surface to the cytoskeleton. We suggest the name cortactin, reflecting the cortical subcellular localization and its actin-binding activity.

MeSH Terms
Actins/isolation & purification,metabolism Amino Acid Sequence Animals Cells, Cultured Chick Embryo Cortactin Glutathione Transferase/genetics,metabolism Kinetics Microfilament Proteins/genetics,isolation & purification,metabolism Molecular Sequence Data Molecular Weight Plasmids Protein Biosynthesis Proto-Oncogene Proteins pp60(c-src)/metabolism Recombinant Fusion Proteins/metabolism Repetitive Sequences, Nucleic Acid Substrate Specificity Transcription, Genetic
Chemicals
Actins Cortactin Microfilament Proteins Recombinant Fusion Proteins Glutathione Transferase Proto-Oncogene Proteins pp60(c-src)
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wu H
Department of Microbiology, University of Virginia Health Sciences Center, Charlottesville 22908.
Parsons J T
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.120.6.1417
Published
1993-03-00
Pages
1417-26
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2119758
Subset
IM
Grants
NCI NIH HHS · CA29243 · United States
NCI NIH HHS · CA40042 · United States
External Links
PubMed source DOI: 10.1083/jcb.120.6.1417 PMC Full Text View journal details
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