Home LiteratureArticle Details
PMID: 7690415 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Cofactor requirement for human immunodeficiency virus type 1 entry into a CD4-expressing human cell line.

Journal of virology ·Vol. 67 ·No. 10 ·1993-10-00 ·Pages 5939-47

Harrington RD, Geballe AP

Abstract

Expression of the human immunodeficiency virus type 1 (HIV-1) receptor CD4 on many nonhuman and some human cell lines is not sufficient to permit HIV-1 infection. We describe a human glioblastoma cell line (U373-MG) which remains resistant to HIV-1 despite the added expression of an authentic CD4 molecule. The block to HIV-1 infection of these cells is strain independent and appears to be at viral entry. Heterokaryons of CD4-expressing U373-MG (U373-CD4) cells fused to HeLa cells allow HIV-1 entry. A U373-CD4/HeLa hybrid clone allows efficient HIV-1 replication. These results suggest that HeLa cells express a factor(s) that can complement the viral entry defect of U373-CD4 cells and is necessary for efficient CD4-mediated HIV-1 infection.

MeSH Terms
Antigens, CD/biosynthesis,isolation & purification,metabolism Base Sequence Blotting, Western CD4 Antigens/biosynthesis,isolation & purification,metabolism Cell Fusion Electrophoresis, Polyacrylamide Gel Flow Cytometry Glioma HIV Reverse Transcriptase HIV-1/physiology HeLa Cells Humans Hybrid Cells Molecular Sequence Data Neoplasm Proteins/isolation & purification,metabolism Oligodeoxyribonucleotides Polymerase Chain Reaction RNA-Directed DNA Polymerase/analysis,metabolism Recombinant Proteins/analysis,biosynthesis Tumor Cells, Cultured Virus Replication beta-Galactosidase/analysis,biosynthesis
Chemicals
Antigens, CD CD4 Antigens Neoplasm Proteins Oligodeoxyribonucleotides Recombinant Proteins HIV Reverse Transcriptase RNA-Directed DNA Polymerase beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Harrington R D
Department of Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104-2092.
Geballe A P
References (34)
34 references, click to expand
  1. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  2. Different requirements for membrane fusion mediated by the envelopes of human immunodeficiency virus types 1 and 2.
    J Virol. 1993 Apr;67(4):2355-9 PMID: 8095307
  3. Detection of a fusion peptide sequence in the transmembrane protein of human immunodeficiency virus.
    Cell. 1987 Jul 31;50(3):327-8 PMID: 3496970
  4. Delineation of a region of the human immunodeficiency virus type 1 gp120 glycoprotein critical for interaction with the CD4 receptor.
    Cell. 1987 Sep 11;50(6):975-85 PMID: 2441877
  5. Functional regions of the envelope glycoprotein of human immunodeficiency virus type 1.
    Science. 1987 Sep 11;237(4820):1351-5 PMID: 3629244
  6. Location and chemical synthesis of a binding site for HIV-1 on the CD4 protein.
    Science. 1988 Jun 3;240(4857):1335-9 PMID: 2453925
  7. Synthetic CD4 peptide derivatives that inhibit HIV infection and cytopathicity.
    Science. 1988 Aug 5;241(4866):712-6 PMID: 2969619
  8. Effects of anti-gp120 monoclonal antibodies on CD4 receptor binding by the env protein of human immunodeficiency virus type 1.
    J Virol. 1988 Oct;62(10):3695-702 PMID: 2458487
  9. Neutralizing antibodies to an immunodominant envelope sequence do not prevent gp120 binding to CD4.
    J Virol. 1988 Nov;62(11):4195-200 PMID: 2845130
  10. Human immunodeficiency virus infection studied in CD4-expressing human-murine T-cell hybrids.
    Virology. 1989 Feb;168(2):267-73 PMID: 2464872
  11. CD4 antigen-based antireceptor peptides inhibit infectivity of human immunodeficiency virus in vitro at multiple stages of the viral life cycle.
    Proc Natl Acad Sci U S A. 1989 Sep;86(18):7139-43 PMID: 2789382
  12. Failure of human immunodeficiency virus entry and infection in CD4-positive human brain and skin cells.
    J Virol. 1990 Jan;64(1):215-21 PMID: 2293663
  13. A CD4 domain important for HIV-mediated syncytium formation lies outside the virus binding site.
    Cell. 1990 Mar 9;60(5):747-54 PMID: 2107024
  14. HIV-1 entry into quiescent primary lymphocytes: molecular analysis reveals a labile, latent viral structure.
    Cell. 1990 Apr 20;61(2):213-22 PMID: 2331748
  15. Novel anti-CD4 monoclonal antibodies separate human immunodeficiency virus infection and fusion of CD4+ cells from virus binding.
    J Exp Med. 1990 Oct 1;172(4):1233-42 PMID: 1698911
  16. HIV-1 tropism for mononuclear phagocytes can be determined by regions of gp120 outside the CD4-binding domain.
    Nature. 1990 Nov 1;348(6296):69-73 PMID: 2172833
  17. Dissociation of gp120 from HIV-1 virions induced by soluble CD4.
    Science. 1990 Nov 23;250(4984):1139-42 PMID: 2251501
  18. Specificity of retroviral RNA packaging.
    J Virol. 1991 Jan;65(1):71-80 PMID: 1985218
  19. Macrophage and T cell-line tropisms of HIV-1 are determined by specific regions of the envelope gp120 gene.
    Nature. 1991 Jan 10;349(6305):167-9 PMID: 1986308
  20. Direct measurement of soluble CD4 binding to human immunodeficiency virus type 1 virions: gp120 dissociation and its implications for virus-cell binding and fusion reactions and their neutralization by soluble CD4.
    J Virol. 1991 Mar;65(3):1133-40 PMID: 1995942
  21. Binding of soluble CD4 proteins to human immunodeficiency virus type 1 and infected cells induces release of envelope glycoprotein gp120.
    Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2189-93 PMID: 2006155
  22. Specific cell surface requirements for the infection of CD4-positive cells by human immunodeficiency virus types 1 and 2 and by Simian immunodeficiency virus.
    Virology. 1991 Apr;181(2):703-15 PMID: 1673040
  23. The V3 loops of the HIV-1 and HIV-2 surface glycoproteins contain proteolytic cleavage sites: a possible function in viral fusion?
    AIDS Res Hum Retroviruses. 1991 Jan;7(1):3-16 PMID: 2015114
  24. Identification of the envelope V3 loop as the primary determinant of cell tropism in HIV-1.
    Science. 1991 Jul 5;253(5015):71-4 PMID: 1905842
  25. Conformational changes induced in the human immunodeficiency virus envelope glycoprotein by soluble CD4 binding.
    J Exp Med. 1991 Aug 1;174(2):407-15 PMID: 1713252
  26. Inhibition of entry of HIV-1 in neural cell lines by antibodies against galactosyl ceramide.
    Science. 1991 Jul 19;253(5017):320-3 PMID: 1857969
  27. Several CD4 domains can play a role in human immunodeficiency virus infection in cells.
    J Virol. 1991 Sep;65(9):4893-901 PMID: 1714523
  28. Stimulation of glycoprotein gp120 dissociation from the envelope glycoprotein complex of human immunodeficiency virus type 1 by soluble CD4 and CD4 peptide derivatives: implications for the role of the complementarity-determining region 3-like region in membrane fusion.
    Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):8082-6 PMID: 1896455
  29. Identification of human immunodeficiency virus envelope gene sequences influencing viral entry into CD4-positive HeLa cells, T-leukemia cells, and macrophages.
    J Virol. 1991 Nov;65(11):5782-9 PMID: 1920616
  30. Detection of replication-competent and pseudotyped human immunodeficiency virus with a sensitive cell line on the basis of activation of an integrated beta-galactosidase gene.
    J Virol. 1992 Apr;66(4):2232-9 PMID: 1548759
  31. A monoclonal antibody to CD4 domain 2 blocks soluble CD4-induced conformational changes in the envelope glycoproteins of human immunodeficiency virus type 1 (HIV-1) and HIV-1 infection of CD4+ cells.
    J Virol. 1992 Aug;66(8):4784-93 PMID: 1378510
  32. Complementation of murine cells for human immunodeficiency virus envelope/CD4-mediated fusion in human/murine heterokaryons.
    J Virol. 1992 Aug;66(8):4794-802 PMID: 1629956
  33. Lack of correlation between soluble CD4-induced shedding of the human immunodeficiency virus type 1 exterior envelope glycoprotein and subsequent membrane fusion events.
    J Virol. 1992 Sep;66(9):5516-24 PMID: 1501286
  34. The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain.
    Cell. 1986 Nov 7;47(3):333-48 PMID: 3094962
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.67.10.5939-5947.1993
Published
1993-10-00
Pages
5939-47
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC238014
Subset
IM
Grants
NIAID NIH HHS · AI27291 · United States
External Links
PubMed source DOI: 10.1128/JVI.67.10.5939-5947.1993 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2