Home LiteratureArticle Details
PMID: 8095307 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Different requirements for membrane fusion mediated by the envelopes of human immunodeficiency virus types 1 and 2.

Journal of virology ·Vol. 67 ·No. 4 ·1993-04-00 ·Pages 2355-9

Dragic T, Alizon M

Abstract

CD4+ cells derived from the human cell lines U87MG and SCL1 cannot be infected by human immunodeficiency virus type 1 (HIV-1) or fuse with cells expressing the HIV-1 envelope. This block was complemented in heterokaryons with HeLa cells and probably reflects the absence of cellular factors necessary for membrane fusion. Since U87MG cells expressing CD4 are permissive to HIV-2, distinct cellular factors could be required for fusion mediated by two related human retroviruses.

MeSH Terms
Animals CD4 Antigens/metabolism CD4-Positive T-Lymphocytes/microbiology Cell Fusion Cells, Cultured Gene Products, env/metabolism HIV-1/pathogenicity HIV-2/pathogenicity HeLa Cells Humans Hybrid Cells In Vitro Techniques Membrane Fusion Mice Receptors, Virus/metabolism Viral Fusion Proteins/physiology
Chemicals
CD4 Antigens Gene Products, env Receptors, Virus Viral Fusion Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dragic T
Institut National de la Santé et de la Recherche Médicale U332, Institut Cochin de Génétique Moléculaire, Paris, France.
Alizon M
References (18)
18 references, click to expand
  1. Genetic variability of the AIDS virus: nucleotide sequence analysis of two isolates from African patients.
    Cell. 1986 Jul 4;46(1):63-74 PMID: 2424612
  2. Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS.
    Science. 1984 May 4;224(4648):497-500 PMID: 6200935
  3. The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain.
    Cell. 1986 Nov 7;47(3):333-48 PMID: 3094962
  4. Genome organization and transactivation of the human immunodeficiency virus type 2.
    Nature. 1987 Apr 16-22;326(6114):662-9 PMID: 3031510
  5. The specificity of the human immunodeficiency virus type 2 transactivator is different from that of human immunodeficiency virus type 1.
    EMBO J. 1987 Dec 1;6(12):3755-60 PMID: 2828036
  6. The human and simian immunodeficiency viruses HIV-1, HIV-2 and SIV interact with similar epitopes on their cellular receptor, the CD4 molecule.
    AIDS. 1988 Apr;2(2):101-5 PMID: 2454642
  7. Failure of human immunodeficiency virus entry and infection in CD4-positive human brain and skin cells.
    J Virol. 1990 Jan;64(1):215-21 PMID: 2293663
  8. Human immunodeficiency virus envelope glycoprotein/CD4-mediated fusion of nonprimate cells with human cells.
    J Virol. 1990 May;64(5):2149-56 PMID: 2109100
  9. Simple methods for monitoring HIV-1 and HIV-2 gp120 binding to soluble CD4 by enzyme-linked immunosorbent assay: HIV-2 has a 25-fold lower affinity than HIV-1 for soluble CD4.
    AIDS. 1990 Apr;4(4):297-305 PMID: 2190604
  10. Cellular factors regulate transactivation of human immunodeficiency virus type 1.
    J Virol. 1991 Mar;65(3):1392-9 PMID: 1995949
  11. Host range mutant of human immunodeficiency virus type 1: modification of cell tropism by a single point mutation at the neutralization epitope in the env gene.
    J Virol. 1991 Apr;65(4):1710-8 PMID: 2002539
  12. Specific cell surface requirements for the infection of CD4-positive cells by human immunodeficiency virus types 1 and 2 and by Simian immunodeficiency virus.
    Virology. 1991 Apr;181(2):703-15 PMID: 1673040
  13. Identification of the envelope V3 loop as the primary determinant of cell tropism in HIV-1.
    Science. 1991 Jul 5;253(5015):71-4 PMID: 1905842
  14. Identification of human immunodeficiency virus envelope gene sequences influencing viral entry into CD4-positive HeLa cells, T-leukemia cells, and macrophages.
    J Virol. 1991 Nov;65(11):5782-9 PMID: 1920616
  15. A second origin of DNA plus-strand synthesis is required for optimal human immunodeficiency virus replication.
    J Virol. 1992 May;66(5):2814-20 PMID: 1560526
  16. Human immunodeficiency virus type 2 infection and fusion of CD4-negative human cell lines: induction and enhancement by soluble CD4.
    J Virol. 1992 Jun;66(6):3531-7 PMID: 1583722
  17. Complementation of murine cells for human immunodeficiency virus envelope/CD4-mediated fusion in human/murine heterokaryons.
    J Virol. 1992 Aug;66(8):4794-802 PMID: 1629956
  18. Isolation of a new human retrovirus from West African patients with AIDS.
    Science. 1986 Jul 18;233(4761):343-6 PMID: 2425430
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.67.4.2355-2359.1993
Published
1993-04-00
Pages
2355-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC240396
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.67.4.2355-2359.1993 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2