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PMID: 7695305 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Kinetic analysis of extension of substrate specificity with Xanthomonas maltophilia, Aeromonas hydrophila, and Bacillus cereus metallo-beta-lactamases.

Antimicrobial agents and chemotherapy ·Vol. 39 ·No. 1 ·1995-01-00 ·Pages 192-9

Felici A, Amicosante G

Abstract

Twenty beta-lactam molecules, including penicillins, cephalosporins, penems, carbapenems, and monobactams, were investigated as potential substrates for Xanthomonas maltophilia ULA-511, Aeromonas hydrophila AE036, and Bacillus cereus 5/B/6 metallo-beta-lactamases. A detailed analysis of the kinetic parameters examined confirmed these enzymes to be broad-spectrum beta-lactamases with different ranges of catalytic efficiency. Cefoxitin and moxalactam, substrates for the beta-lactamases from X. maltophilia ULA-511 and B. cereus 5/B/6, behaved as inactivators of the A. hydrophila AE036 metallo-beta-lactamase, which appeared to be unique among the enzymes tested in this study. In addition, we report a new, faster, and reliable purification procedure for the B. cereus 5/B/6 metallo-beta-lactamase, cloned in Escherichia coli HB101.

MeSH Terms
Aeromonas hydrophila/drug effects Anti-Bacterial Agents/chemistry,pharmacology Bacillus cereus/drug effects Kinetics Molecular Structure Substrate Specificity Xanthomonas/drug effects beta-Lactamase Inhibitors beta-Lactamases/isolation & purification beta-Lactams
Chemicals
Anti-Bacterial Agents beta-Lactamase Inhibitors beta-Lactams beta-Lactamases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Felici A
Dipartimento di Scienze e Tecnologie Biomediche e di Biometria, Università degli Studi dell'Aquila, Italy.
Amicosante G
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.39.1.192
Published
1995-01-00
Pages
192-9
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC162508
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.39.1.192 PMC Full Text View journal details
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