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PMID: 7708735 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Krebs cycle function is required for activation of the Spo0A transcription factor in Bacillus subtilis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 7 ·1995-03-28 ·Pages 2845-9

Ireton K, Jin S, Grossman AD, Sonenshein AL

Abstract

Expression of genes early during sporulation in Bacillus subtilis requires the activity of the transcription factor encoded by spo0A. The active, phosphorylated form of Spo0A is produced through the action of a multicomponent pathway, the phosphorelay. A mutant defective in the first three enzymes of the Krebs citric acid cycle was unable to express early sporulation genes, apparently because of a failure to activate the phosphorelay. Cells that produce an altered Spo0A protein that can be phosphorylated by an alternative pathway were not dependent on Krebs cycle function for early sporulation gene expression. These findings suggest that Krebs cycle enzymes transmit a signal to activate the phosphorelay and that B. subtilis monitors its metabolic potential before committing itself to spore formation.

Related Genes
cit citA citC citZ sof-1 spo0A
MeSH Terms
Bacillus subtilis/growth & development,physiology Bacterial Proteins/biosynthesis Citric Acid Cycle Gene Deletion Gene Expression Genotype Glucose/metabolism Kinetics Mutagenesis, Insertional Phosphorylation Promoter Regions, Genetic Spores, Bacterial/physiology Transcription Factors/biosynthesis
Chemicals
Bacterial Proteins Spo0A protein, Bacillus subtilis Transcription Factors Glucose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ireton K
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
Jin S
Grossman A D
Sonenshein A L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.7.2845
Published
1995-03-28
Pages
2845-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC42315
Subset
IM
Grants
NIGMS NIH HHS · GM36718 · United States
NIGMS NIH HHS · GM41934 · United States
NIGMS NIH HHS · GM42219 · United States
External Links
PubMed source DOI: 10.1073/pnas.92.7.2845 PMC Full Text View journal details
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