Cloning, nucleotide sequence, and transcriptional analysis of the nusG gene of Streptomyces coelicolor A3(2), which encodes a putative transcriptional antiterminator.

Molecular & general genetics : MGG ·Vol. 247 ·No. 1 ·1995-04-10 ·Pages 118-22

Puttikhunt C, Nihira T, Yamada Y

Abstract

A 3 kb genomic fragment containing the nusG gene of Streptomyces coelicolor A3(2) was identified, cloned and sequenced. Sequence analysis revealed 3 complete and 2 truncated open reading frames (ORFs): truncated ORFU (similar to a Bacillus gene encoding a thermostable aspartate aminotransferase)-secE (94 amino acids; 79.0% similarity to Escherichia coli SecE)-nusG (300 amino acids; 73.3% similarity to E. coli NusG)-rplK (144 amino acids; 88.5% similarity to E. coli ribosomal subunit L11)-truncated rplA (similar to E. coli ribosomal subunit L1). The gene organization secE-nusG-rplKA exactly matches that in E. coli. Transcriptional analyses by the primer extension method revealed one transcriptional start site each for secE and nusG, and two sites for rplK. The presence of promoters was also confirmed with the aid of a promoter-probe vector.

Related Genes

nusG rplK secE

MeSH Terms

Amino Acid Sequence Bacterial Proteins/chemistry,genetics Base Sequence Cloning, Molecular DNA, Bacterial/genetics Escherichia coli Proteins Genes, Bacterial Molecular Sequence Data Open Reading Frames Peptide Elongation Factors/chemistry,genetics Promoter Regions, Genetic Restriction Mapping Sequence Analysis, DNA Streptomyces/genetics Terminator Regions, Genetic Transcription Factors/biosynthesis,chemistry,genetics Transcription, Genetic

Chemicals

Bacterial Proteins DNA, Bacterial Escherichia coli Proteins NusG protein, E coli Peptide Elongation Factors Transcription Factors

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Puttikhunt C

Department of Biotechnology, Faculty of Engineering, Osaka University, Japan.

Nihira T

Yamada Y

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22 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00425829

Published

1995-04-10

Pages

118-22

Language

English

Region

Germany

NLM ID

0125036

Subset

Databases

GENBANK

D32254

External Links

PubMed source DOI: 10.1007/BF00425829

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Cloning, nucleotide sequence, and transcriptional analysis of the nusG gene of Streptomyces coelicolor A3(2), which encodes a putative transcriptional antiterminator.

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