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PMID: 7720708 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Inactivation of Myf-6 and Myf-5 genes in mice leads to alterations in skeletal muscle development.

The EMBO journal ·Vol. 14 ·No. 6 ·1995-03-15 ·Pages 1176-86

Braun T, Arnold HH

Abstract

Myf-6, alternatively called MRF4 or herculin, is a member of a group of muscle-specific transcription factors which also comprises Myf-5, myogenin and MyoD. All family members show distinct expression patterns during skeletal muscle development and can convert a variety of cell lines to myocytes. We disrupted the Myf-6 gene in mice to investigate its functional role in the network of regulatory factors controlling myogenesis. Homozygous mice carrying the disrupted Myf-6 gene show pronounced down-regulation of Myf-5 transcription for reasons presently unknown. Consequently, these mice represent a double knock-out model for Myf-6 and Myf-5. The mutants resemble most of the Myf-5 phenotype with aberrant and delayed early myotome formation and lack of distal rib structures. In addition, we find a reduction in the size of axial muscles in the back. Apart from changes in the pattern of some contractile protein isoforms, the existing myofibers appear fairly normal. This suggests that Myf-6 has no major role in the maturation of myotubes, as previously proposed. Our results provide evidence that skeletal myogenesis can proceed in the absence of two myogenic factors, Myf-5 and Myf-6, therefore they must exert largely non-redundant functions in vivo.

Related Genes
Myf-5 Myf-6
MeSH Terms
Animals DNA-Binding Proteins Gene Expression Regulation, Developmental/physiology Gene Targeting Genes, Lethal Homozygote Mice Mice, Knockout Muscle Development Muscle Fibers, Skeletal/physiology Muscle Proteins/genetics,physiology Muscle, Skeletal/cytology,embryology,growth & development Myogenic Regulatory Factor 5 Myogenic Regulatory Factors/genetics,physiology Myogenin RNA, Messenger/analysis Ribs/growth & development Trans-Activators Transcription Factors/genetics,physiology
Chemicals
DNA-Binding Proteins Muscle Proteins Myf5 protein, mouse Myog protein, mouse Myogenic Regulatory Factor 5 Myogenic Regulatory Factors Myogenin RNA, Messenger Trans-Activators Transcription Factors myogenic factor 6
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Braun T
Department of Cell and Molecular Biology, University of Braunschweig, Germany.
Arnold H H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-03-15
Pages
1176-86
Language
English
Region
England
NLM ID
8208664
PMCID
PMC398195
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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