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PMID: 7721701 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification and sequence analysis of lpfABCDE, a putative fimbrial operon of Salmonella typhimurium.

Journal of bacteriology ·Vol. 177 ·No. 8 ·1995-04-00 ·Pages 2087-97

Bäumler AJ, Heffron F

Abstract

A chromosomal region present in Salmonella typhimurium but absent from related species was identified by hybridization. A DNA probe originating from 78 min on the S. typhimurium chromosome hybridized with DNA from Salmonella enteritidis, Salmonella heidelberg, and Salmonella dublin but not with DNA from Salmonella typhi, Salmonella arizonae, Escherichia coli, and Shigella serotypes. Cloning and sequence analysis revealed that the corresponding region of the S. typhimurium chromosome encodes a fimbrial operon. Long fimbriae inserted at the poles of the bacterium were observed by electron microscopy when this fimbrial operon was introduced into a nonpiliated E. coli strain. The genes encoding these fimbriae were therefore termed lpfABCDE, for long polar fimbriae. Genetically, the lpf operon was found to be most closely related to the fim operon of S. typhimurium, both in gene order and in conservation of the deduced amino acid sequences.

Related Genes
lpfABCDE
MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Chromosome Mapping Cloning, Molecular DNA, Bacterial/genetics Escherichia coli/genetics Fimbriae, Bacterial/metabolism Genes, Bacterial Molecular Sequence Data Operon Promoter Regions, Genetic Repetitive Sequences, Nucleic Acid Salmonella/genetics Salmonella typhimurium/genetics,metabolism,ultrastructure Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Species Specificity
Chemicals
Bacterial Proteins DNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bäumler A J
Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland 97201-3098, USA.
Heffron F
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.8.2087-2097.1995
Published
1995-04-00
Pages
2087-97
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC176853
Subset
IM
Grants
NIAID NIH HHS · R0I AI 22933 · United States
Databases
GENBANK
U18559
External Links
PubMed source DOI: 10.1128/jb.177.8.2087-2097.1995 PMC Full Text View journal details
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