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PMID: 7910820 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Proteus mirabilis MR/P fimbrial operon: genetic organization, nucleotide sequence, and conditions for expression.

Journal of bacteriology ·Vol. 176 ·No. 11 ·1994-06-00 ·Pages 3412-9

Bahrani FK, Mobley HL

Abstract

Proteus mirabilis, an agent of urinary tract infection, expresses at least four fimbrial types. Among these are the MR/P (mannose-resistant/Proteus-like) fimbriae. MrpA, the structural subunit, is optimally expressed at 37 degrees C in Luria broth cultured statically for 48 h by each of seven strains examined. Genes encoding this fimbria were isolated, and the complete nucleotide sequence was determined. The mrp gene cluster encoded by 7,293 bp predicts eight polypeptides: MrpI (22,133 Da), MrpA (17,909 Da), MrpB (19,632 Da), MrpC (96,823 Da), MrpD (27,886 Da), MrpE (19,470 Da), MrpF (17,363 Da), and MrpG (13,169 Da). mrpI is upstream of the gene encoding the major structural subunit gene mrpA and is transcribed in the direction opposite to that of the rest of the operon. All predicted polypeptides share > or = 25% amino acid identity with at least one other enteric fimbrial gene product encoded by the pap, fim, smf, fan, or mrk gene clusters.

Related Genes
mrpA mrpB mrpC mrpD mrpE mrpF mrpG mrpI
MeSH Terms
Amino Acid Sequence Bacterial Proteins/biosynthesis,genetics Base Sequence Fimbriae, Bacterial/physiology Gene Expression Regulation, Bacterial Genes, Bacterial/genetics Molecular Sequence Data Multigene Family/genetics Operon/genetics Proteus mirabilis/genetics Sequence Analysis, DNA Sequence Homology, Amino Acid Transcription, Genetic
Chemicals
Bacterial Proteins mrpA protein, Proteus mirabilis
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bahrani F K
Department of Medicine, University of Maryland School of Medicine, Baltimore 21201.
Mobley H L
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.11.3412-3419.1994
Published
1994-06-00
Pages
3412-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205519
Subset
IM
Grants
NIA NIH HHS · AG4393 · United States
NIAID NIH HHS · AI23328 · United States
Databases
GENBANK
Z32686
External Links
PubMed source DOI: 10.1128/jb.176.11.3412-3419.1994 PMC Full Text View journal details
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