Proteolytic processing is required for viral superantigen activity.
The mouse mammary tumor virus-7 superantigen (vSAG7) is proteolytically processed in B cells at as many as three positions. Proteolytic processing appears to be important for superantigen activity because a processed form of vSAG7 was predominant among those forms that were found to bind to major histocompatibility complex class II molecules. To determine the functional significance of proteolytic processing, a mutation was introduced in vSAG7 at one of the sites where proteolytic cleavage is thought to take place in B cells. Elimination of the putative processing site at position 171 abrogated detectable vSAG7 surface expression in B cells, indicating that proteolytic processing is required for vSAG7 function. Coexpression in insect cells of vSAG7 and furin, a proprotein-processing enzyme, also demonstrated that furin could process vSAG7 at position 171.
25 references, click to expand
-
Structure and expression of mouse furin, a yeast Kex2-related protease. Lack of processing of coexpressed prorenin in GH4C1 cells.J Biol Chem. 1990 Dec 25;265(36):22075-8 PMID: 2266110
-
Residues of the variable region of the T-cell-receptor beta-chain that interact with S. aureus toxin superantigens.Nature. 1990 Aug 2;346(6283):471-3 PMID: 2377208
-
Epitope tagging and protein surveillance.Methods Enzymol. 1991;194:508-19 PMID: 1706460
-
Cloning and primary sequence of a mouse candidate prohormone convertase PC1 homologous to PC2, Furin, and Kex2: distinct chromosomal localization and messenger RNA distribution in brain and pituitary compared to PC2.Mol Endocrinol. 1991 Jan;5(1):111-22 PMID: 2017186
-
Mammalian subtilisins: the long-sought dibasic processing endoproteases.Cell. 1991 Jul 12;66(1):1-3 PMID: 2070411
-
Mutations within the proteolytic cleavage site of the Rous sarcoma virus glycoprotein define a requirement for dibasic residues for intracellular cleavage.J Virol. 1992 Feb;66(2):865-74 PMID: 1370559
-
Preferred sequence requirements for cleavage of pro-von Willebrand factor by propeptide-processing enzymes.Blood. 1992 May 1;79(9):2349-55 PMID: 1571548
-
Activation of human furin precursor processing endoprotease occurs by an intramolecular autoproteolytic cleavage.J Biol Chem. 1992 Jul 15;267(20):14304-8 PMID: 1629222
-
Detection and biochemical characterization of the mouse mammary tumor virus 7 superantigen (Mls-1a).Cell. 1992 Nov 27;71(5):719-30 PMID: 1330323
-
The new enzymology of precursor processing endoproteases.J Biol Chem. 1992 Nov 25;267(33):23435-8 PMID: 1429684
-
Inhibition of furin-mediated cleavage activation of HIV-1 glycoprotein gp160.Nature. 1992 Nov 26;360(6402):358-61 PMID: 1360148
-
Direct evidence for the role of COOH terminus of mouse mammary tumor virus superantigen in determining T cell receptor V beta specificity.J Exp Med. 1993 Aug 1;178(2):737-41 PMID: 7688034
-
Characterization of a secreted form of human furin endoprotease.Biochem Biophys Res Commun. 1993 Sep 15;195(2):1011-8 PMID: 8373381
-
Superantigens and their potential role in human disease.Adv Immunol. 1993;54:99-166 PMID: 8397479
-
Superantigens: bacterial and viral proteins that manipulate the immune system.Annu Rev Cell Biol. 1993;9:101-28 PMID: 7506550
-
Intracellular trafficking and activation of the furin proprotein convertase: localization to the TGN and recycling from the cell surface.EMBO J. 1994 Jan 1;13(1):18-33 PMID: 7508380
-
Production of soluble MHC class II proteins with covalently bound single peptides.Nature. 1994 May 12;369(6476):151-4 PMID: 8177320
-
Processing and major histocompatibility complex binding of the MTV7 superantigen.Immunity. 1994 Apr;1(1):23-33 PMID: 7889395
-
Antigen-induced lymphomagenesis: identification of a murine B cell lymphoma with known antigen specificity.J Immunol. 1983 Oct;131(4):2064-8 PMID: 6194226
-
A human beta-actin expression vector system directs high-level accumulation of antisense transcripts.Proc Natl Acad Sci U S A. 1987 Jul;84(14):4831-5 PMID: 2440031
-
fur gene expression as a discriminating marker for small cell and nonsmall cell lung carcinomas.J Clin Invest. 1987 Dec;80(6):1545-9 PMID: 2824565
-
Endoproteolytic cleavage of gp160 is required for the activation of human immunodeficiency virus.Cell. 1988 Apr 8;53(1):55-67 PMID: 2450679
-
The molecular biology of influenza virus pathogenicity.Adv Virus Res. 1988;34:247-81 PMID: 3046255
-
Expression and characterization of a functional human immunodeficiency virus envelope glycoprotein in insect cells.Virology. 1990 Jun;176(2):575-86 PMID: 2111957
-
Identification of a cDNA encoding a second putative prohormone convertase related to PC2 in AtT20 cells and islets of Langerhans.Proc Natl Acad Sci U S A. 1991 Jan 15;88(2):340-4 PMID: 1988934
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