Home LiteratureArticle Details
PMID: 772677 Published · ppublish English Journal Article

Involvement of escherichia coli dnaZ gene product in DNA elongation in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 4 ·1976-04-00 ·Pages 1053-7

Wickner S, Hurwitz J

Abstract

E. coli dnaZ gene product is required for conversion of phiX174, fd, and ST-1 single-stranded phage DNAs to duplex DNAs in vitro. This protein has been purified about 5000-fold. It functions in the elongation of RNA- or DNA-primed single-stranded DNA that is catalyzed by DNA polymerase III(DNA nucleotidyltransferase; deoxynucleosidetriphosphate: DNA deoxynucleotidyltransferase; EC 2.7.7.7) in conjunctions with two other E. coli protein preparations referred to as DNA elongation factors I and III. It also functions in similar reactions catalyzed by DNA polymerase II in combination with E. coli DNA binding protein and DNA elongation factors I and III.

MeSH Terms
Bacterial Proteins/isolation & purification,metabolism Cell-Free System DNA Nucleotidyltransferases/metabolism DNA Replication DNA, Bacterial/biosynthesis DNA, Single-Stranded/metabolism Escherichia coli/metabolism Genes
Chemicals
Bacterial Proteins DNA, Bacterial DNA, Single-Stranded DNA Nucleotidyltransferases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wickner S
Hurwitz J
References (23)
23 references, click to expand
  1. Deoxyribonucleic acid plymerase II. of Escherichia coli. I. The purification and characterization of the enzyme.
    J Biol Chem. 1972 Jan 25;247(2):489-97 PMID: 4333263
  2. Conversion of the M13 viral single strand to the double-stranded replicative forms by purified proteins.
    J Biol Chem. 1974 Jul 10;249(13):3999-4005 PMID: 4604274
  3. Multienzyme systems of DNA replication.
    Science. 1974 Dec 13;186(4168):987-93 PMID: 4620044
  4. Studies on in vitro DNA synthesis. Isolation of DNA B gene product from Escherichia coli.
    Proc Natl Acad Sci U S A. 1973 Nov;70(11):3120-4 PMID: 4274130
  5. Replication of phiA and phiX174 in Escherichia coli mutants thermosensitive in DNA synthesis.
    Mol Gen Genet. 1975 Sep 8;139(4):285-91 PMID: 1102942
  6. dnaG gene product, a rifampicin-resistant RNA polymerase, initiates the conversion of a single-stranded coliphage DNA to its duplex replicative form.
    J Biol Chem. 1975 Aug 10;250(15):5995-6001 PMID: 1097446
  7. Conversion of phiX174 viral DNA to double-stranded form by purified Escherichia coli proteins.
    Proc Natl Acad Sci U S A. 1974 Oct;71(10):4120-4 PMID: 4610569
  8. A holoenzyme form of deoxyribonucleic acid polymerase III. Isolation and properties.
    J Biol Chem. 1974 Oct 10;249(19):6244-9 PMID: 4608499
  9. Growth of phages lambda, phiX174, and Ml3 requires the dnaZ (previously dnaH) gene product of Escherichia coli.
    Biochem Biophys Res Commun. 1974 Dec 11;61(3):1036-42 PMID: 4615710
  10. Bacterial cell division regulation: characterization of the dnaH locus of Escherichia coli.
    J Bacteriol. 1974 Aug;119(2):443-9 PMID: 4604144
  11. Purification and properties of DNA polymerase 3.
    Eur J Biochem. 1973 May 2;34(3):440-7 PMID: 4577195
  12. A new form of DNA polymerase 3 and a copolymerase replicate a long, single-stranded primer-template.
    Proc Natl Acad Sci U S A. 1973 Jun;70(6):1764-7 PMID: 4578443
  13. Involvement of two protein factors and ATP in in vitro DNA synthesis catalyzed by DNA polymerase 3 of Escherichia coli.
    Proc Natl Acad Sci U S A. 1974 Jan;71(1):6-10 PMID: 4589895
  14. Studies on in vitro DNA synthesis. II. Isolation of a protein which stimulates deoxynucleotide incorporation catalyzed by DNA polymerase of E. coli.
    Biochem Biophys Res Commun. 1973 Mar 17;51(2):257-67 PMID: 4571401
  15. A DNA-unwinding protein isolated from Escherichia coli: its interaction with DNA and with DNA polymerases.
    Proc Natl Acad Sci U S A. 1972 Dec;69(12):3537-41 PMID: 4566449
  16. Conversion of phiX174 and fd single-stranded DNA to replicative forms in extracts of Escherichia coli.
    Proc Natl Acad Sci U S A. 1972 Nov;69(11):3233-7 PMID: 4564209
  17. RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form.
    Proc Natl Acad Sci U S A. 1972 Apr;69(4):965-9 PMID: 4554537
  18. Initiation of DNA synthesis: synthesis of phiX174 replicative form requires RNA synthesis resistant to rifampicin.
    Proc Natl Acad Sci U S A. 1972 Sep;69(9):2691-5 PMID: 4560696
  19. DNA polymerase 3 star requires ATP to start synthesis on a primed DNA.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3679-83 PMID: 4519657
  20. Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis.
    Proc Natl Acad Sci U S A. 1971 Dec;68(12):3150-3 PMID: 4943556
  21. Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase.
    J Biol Chem. 1969 Jun 10;244(11):2996-3008 PMID: 4890762
  22. Deoxyribonucleic acid polymerase III of Escherichia coli. Purification and properties.
    J Biol Chem. 1975 Jan 25;250(2):461-9 PMID: 1089643
  23. Interaction of Escherichia coli dnaB and dnaC(D) gene products in vitro.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):921-5 PMID: 1093174
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.4.1053
Published
1976-04-00
Pages
1053-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430198
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.4.1053 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2