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PMID: 7726900 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Generation and characterization of an ordered lambda clone array for the 460-kb region surrounding the murine Xist sequence.

Mammalian genome : official journal of the International Mammalian Genome Society ·Vol. 5 ·No. 7 ·1994-07-00 ·Pages 416-23

Rougeulle C, Colleaux L, Dujon B, Avner P

Abstract

The Xist sequence has several characteristics that make it a potential candidate for the X-inactivation center. To investigate the role of Xist and adjacent sequences lying within the X-inactivation center candidate region, a 460-kb region surrounding the murine Xist sequence has been arrayed in lambda contigs with a combination of IRS-PCR-based hybridization and YAC fragmentation. The orientation of the Xist sequence in relation to the telomere and centromere of the X Chromosome (Chr) has been established with this contig and shown to be inverted compared to that in human.

Related Genes
Xist
MeSH Terms
Animals Bacteriophage lambda/genetics Base Sequence Chromosomes, Artificial, Yeast Cloning, Molecular Dosage Compensation, Genetic Female Male Mice Mice, Inbred C3H Mice, Transgenic Molecular Sequence Data Oligodeoxyribonucleotides Polymerase Chain Reaction RNA, Long Noncoding RNA, Untranslated Transcription Factors/genetics X Chromosome
Chemicals
Oligodeoxyribonucleotides RNA, Long Noncoding RNA, Untranslated Transcription Factors XIST non-coding RNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rougeulle C
Unité de Génétique Moléculaire Murine, Institut Pasteur, Paris, France.
Colleaux L
Dujon B
Avner P
References (24)
24 references, click to expand
  1. Nested chromosomal fragmentation in yeast using the meganuclease I-Sce I: a new method for physical mapping of eukaryotic genomes.
    Nucleic Acids Res. 1992 Nov 11;20(21):5625-31 PMID: 1333585
  2. Genomic sequencing.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 PMID: 6326095
  3. Differential distribution of long and short interspersed element sequences in the mouse genome: chromosome karyotyping by fluorescence in situ hybridization.
    Proc Natl Acad Sci U S A. 1990 Oct;87(19):7757-61 PMID: 2170987
  4. Extension of the physical map in the region of the mouse X chromosome homologous to human Xq28 and identification of an exception to conserved linkage.
    Genomics. 1992 Aug;13(4):1289-95 PMID: 1354645
  5. Generation of deletion derivatives by targeted transformation of human-derived yeast artificial chromosomes.
    Proc Natl Acad Sci U S A. 1990 Feb;87(4):1300-4 PMID: 2406718
  6. Yeast artificial chromosome libraries containing large inserts from mouse and human DNA.
    Proc Natl Acad Sci U S A. 1991 May 15;88(10):4123-7 PMID: 2034658
  7. Rapid assembly of lambda phage contigs within YAC clones.
    Nucleic Acids Res. 1991 May 25;19(10):2795-6 PMID: 1828295
  8. The human XIST gene: analysis of a 17 kb inactive X-specific RNA that contains conserved repeats and is highly localized within the nucleus.
    Cell. 1992 Oct 30;71(3):527-42 PMID: 1423611
  9. Distribution of Alu and L1 repeats in human YAC recombinants.
    Mamm Genome. 1992;3(12):661-8 PMID: 1477474
  10. The product of the mouse Xist gene is a 15 kb inactive X-specific transcript containing no conserved ORF and located in the nucleus.
    Cell. 1992 Oct 30;71(3):515-26 PMID: 1423610
  11. Conservation and reorganization of loci on the mammalian X chromosome: a molecular framework for the identification of homologous subchromosomal regions in man and mouse.
    Genomics. 1988 Apr;2(3):220-30 PMID: 2899541
  12. A gene from the region of the human X inactivation centre is expressed exclusively from the inactive X chromosome.
    Nature. 1991 Jan 3;349(6304):38-44 PMID: 1985261
  13. Mapping the murine Xce locus with (CA)n repeats.
    Mamm Genome. 1993 Sep;4(9):523-30 PMID: 8118102
  14. Genetic and molecular evidence of an X-chromosome deletion spanning the tabby (Ta) and testicular feminization (Tfm) loci in the mouse.
    Cytogenet Cell Genet. 1991;56(3-4):137-43 PMID: 2055107
  15. Adaptation of the interspersed repetitive sequence polymerase chain reaction to the isolation of mouse DNA probes from somatic cell hybrids on a hamster background.
    Genomics. 1991 Jul;10(3):770-8 PMID: 1889819
  16. Physical mapping and YAC contig analysis of the region surrounding Xist on the mouse X chromosome.
    Genomics. 1993 Mar;15(3):559-69 PMID: 8468050
  17. Mapping of the distal boundary of the X-inactivation center in a rearranged X chromosome from a female expressing XIST.
    Hum Mol Genet. 1993 Jul;2(7):883-7 PMID: 8364571
  18. Rapid physical mapping of YAC inserts by random integration of I-Sce I sites.
    Hum Mol Genet. 1993 Mar;2(3):265-71 PMID: 8499915
  19. Conservation of position and exclusive expression of mouse Xist from the inactive X chromosome.
    Nature. 1991 May 23;351(6324):329-31 PMID: 2034279
  20. Combinatoric strategies for genome mapping.
    Bioessays. 1991 Jan;13(1):39-44 PMID: 1772409
  21. X-chromosome deletions in embryo-derived (EK) cell lines associated with lack of X-chromosome inactivation.
    J Embryol Exp Morphol. 1985 Dec;90:379-88 PMID: 3834036
  22. Non-random X-chromosome inactivation in mouse X-autosome translocation embryos--location of the inactivation centre.
    J Embryol Exp Morphol. 1983 Dec;78:1-22 PMID: 6198418
  23. Characterization of a murine gene expressed from the inactive X chromosome.
    Nature. 1991 May 23;351(6324):325-9 PMID: 2034278
  24. Metaphase chromosome transfer of introduced selectable markers.
    J Mol Appl Genet. 1984;2(6):563-77 PMID: 6099401
Article Info
Journal
Mammalian genome : official journal of the International Mammalian Genome Society
Abbr.
Mamm Genome
ISSN
0938-8990
DOI
10.1007/BF00357001
Published
1994-07-00
Pages
416-23
Language
English
Region
United States
NLM ID
9100916
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1007/BF00357001 View journal details
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