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PMID: 7727749 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The Pisum sativum TubA1 gene, a member of a small family of alpha-tubulin sequences.

Plant molecular biology ·Vol. 27 ·No. 4 ·1995-02-00 ·Pages 715-27

Brierley HL, Webster P, Long SR

Abstract

alpha- and beta-tubulin proteins are subunits of microtubules, which as primary elements of the plant cytoskeleton play major roles in plant cell division and cell morphogenesis. Several higher-plant alpha- and beta-tubulin gene families have been reported to have at least six to nine members each. Using genomic Southern hybridizations and polymerase chain reaction (PCR) experiments, we have found that the Pisum sativum (garden pea) genome has only four copies of alpha-tubulin sequences and a similar number of beta-tubulin sequences. We have characterized the pea alpha-tubulin gene TubA1. Its nucleotide sequence predicts a 452 amino acid product which is 89-98% identical to those predicted for other plant alpha-tubulins. By S1 nuclease analysis we have located the transcript start site at 102 bases upstream of the ATG. We have also shown that the TubA1 gene is expressed by northern hybridization with a gene-specific probe.

Related Genes
TubA1
MeSH Terms
Base Sequence DNA Probes Gene Expression Regulation, Plant Genes, Plant Molecular Sequence Data Peas/genetics Tubulin/genetics
Chemicals
DNA Probes Tubulin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brierley H L
Department of Biological Sciences, Stanford University, CA 94305-5020, USA.
Webster P
Long S R
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00020225
Published
1995-02-00
Pages
715-27
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
U12589
External Links
PubMed source DOI: 10.1007/BF00020225 View journal details
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